Increased calcium in carrots by expression of an Arabidopsis H+/Ca2+ transporter

被引:0
作者
Sunghun Park
Chang-Kil Kim
Leonard M. Pike
Roberta H. Smith
Kendal D. Hirschi
机构
[1] Texas A&M University,Vegetable and Fruit Improvement Center
[2] College Station,Dept. of Horticulture
[3] Sang-Ju National University,Baylor College of Medicine, Depts of Pediatrics, and Human and Molecular Genetics
[4] USDA/ARS Children's Nutrition Research Center,undefined
来源
Molecular Breeding | 2004年 / 14卷
关键词
Calcium; Carrot; H; /Ca; transporter; Nutrition;
D O I
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中图分类号
学科分类号
摘要
We demonstrate that carrots expressing the Arabidopsis H+/Ca2+ transporter CAX1 (Cation Exchanger 1) contained up to 50% more calcium (Ca) than plants transformed with control vectors. The CAX1-expressing carrots were fertile, and robust plant growth was seen in the majority of the transgenic plants. CAX1-expressing carrots were crossed to a commercial carrot variety to confirm that the increased Ca accumulation was mediated by CAX1-expression, and the increased Ca content was clearly correlated with the transgene. This study suggests that modulation of ion transporters could be an important means of increasing the Ca content of agriculturally important crops. To our knowledge, this study represents the first attempts to use biotechnology to increase the Ca content of an agriculturally important crop.
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页码:275 / 282
页数:7
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共 55 条
  • [1] Bachrach L.K.(2001)Acquisition of optimal bone mass in child-hood and adolescence Trends. Endocrin. Met. 12 22-28
  • [2] Bryant R.J.(1999)The new dietary reference intakes for calcium: Implications for osteoporosis J. Am. College of Nutr. 18 406S-412S
  • [3] Cadogan J.(1996)Control of root growth and development by cyclin expression Nature 380 520-523
  • [4] Weaver C.M.(1955)Multiple range and multiple F tests Biomet-rics 11 1-42
  • [5] Doerner P.(1994)Papermill sludge, phosphorous, potassium, and lime effect on clover grown on a mine soil J. Environ Qual. 23 759-765
  • [6] Jorgensen J-E.(1996)Intrinsic Ca-Labelling of Green Bean Pods for Use in Human Bioavailability Studies J. Sci. Food Agric. 70 11-15
  • [7] You R.(2001)Vacuolar H Trends Plant Sci. 6 100-104
  • [8] Steppuhn J.(1999) /Ca 2 Plant Cell 11 2113-2122
  • [9] Lamb C.(1983) transport: Who's directing the traffic? Nature 303 179-180
  • [10] Duncan D.B.(1978)Expression of Mol. Gen. Genet. 163 181-187