Protein-ligand binding affinity determination by the waterLOGSY method: An optimised approach considering ligand rebinding

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作者：
Renjie Huang
Arnaud Bonnichon
Timothy D. W. Claridge
Ivanhoe K. H. Leung
机构：
[1] School of Chemical Sciences,Department of Chemistry
[2] The University of Auckland,undefined
[3] Private Bag 92019,undefined
[4] University of Oxford,undefined
[5] Chemistry Research Laboratory,undefined
[6] Université D’Auvergne,undefined
[7] 49 Boulevard François-Mitterrand,undefined
[8] CS 60032,undefined
来源：
Scientific Reports | / 7卷
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摘要：
WaterLOGSY is a popular ligand-observed NMR technique to screen for protein-ligand interactions, yet when applied to measure dissociation constants (KD) through ligand titration, the results were found to be strongly dependent on sample conditions. Herein, we show that accurate KDs can be obtained by waterLOGSY with optimised experimental setup.
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