Cloning and expression of rat transforming growth factor beta 1 cDNA in osteoblasts

被引：0

作者：

Yong L. ^{[1
]}

Qixin Z. ^{[1
]}

Jingyuan D. ^{[1
]}

Hui Z. ^{[1
]}

Xiaodong G. ^{[1
]}

Shen Q. ^{[2
]}

机构：

[1] Department of Orthopaedics, Xiehe Hospital, Tongji Medical University, Wuhan

[2] Department of Biochemistry, School of Basic Medical Sciences, Tongji Medical University, Wuhan

来源：

Current Medical Science | 2000年 / 20卷 / 1期

关键词：

transforming growth factor β1; gene expression; RT-PCR; osteoblasts; molecular cloning;

D O I：

10.1007/BF02887680

中图分类号：

学科分类号：

摘要：

Rat transforming growth factor beta 1 (rTGF beta 1) cDNA from rat lymphocytes was cloned by RT-PCR and inserted into pcDNA3 to construct an eukaryotic expression vector, which was named pcDNA3-TGF beta 1. The cloned gene was confirmed to code rat TGF beta 1 by restriction enzyme analysis. pcDNA3-TGF beta 1 plasmid was transfected into rat osteoblasts by using liposome-mediated gene transfer technique and the expression of TGF beta 1 was detected by using immunohistochemical staining assay. It was found that the rat TGF beta 1 expression product was obviously detectable in the transfected osteoblasts in 48 h. High expression of TGF beta 1 was obtained in the rat osteoblasts in which the constructed TGF beta 1 expression vector was transfected.

引用

页码：63 / 65

页数：2

共 9 条

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[2]

Kondaiah P(1994)Local injection of TGF-beta increases the strength of tibial fractures in the rat Acta Orthop Scand 65 37-37

[3]

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[4]

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