Magnetic polysiloxane-polyvinyl alcohol composite as solid-phase in chemiluminescent assays

被引：0
作者：
R.A.L. Coêlho
G.A. Jaques
A.D. Barbosa
G. Velazquez
S.M.L. Montenegro
W.M. Azevedo
L.B. Carvalho
机构：
[1] Universidade Federal de Pernambuco,Laboratório de Imunopatologia Keizo Asami and Departamento de Bioquímica
[2] Universidad Nacional de Asuncion,Instituto de Investigaciones en Ciencias de la Salud
[3] FIOCRUZ,Centro de Pesquisas Aggeu Magalhães
[4] Universidade Federal de Pernambuco,Departamento de Química Fundamental
来源：
Biotechnology Letters | 2002年 / 24卷
关键词：
chemiluminescence; polysiloxane; polyvinyl alcohol; solid support;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A polysiloxane and polyvinyl alcohol interpenetrating polymer network was synthesised and its ferromagnetic derivative was used as solid support for antigen covalent immobilisation in chemiluminescent assays. Only 0.625 μg of either Trypanosoma cruzi or Schistosoma mansoni antigens immobilized onto the magnetic particles (2.5 mg) were sufficient to detect the correspondent human IgG within a nanogram scale.
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页码：1705 / 1708
页数：3
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