The Mutant Form of Acetolactate Synthase Genomic DNA from Rice is an Efficient Selectable Marker for Genetic Transformation

被引:0
|
作者
Keishi Osakabe
Masaki Endo
Kiyoshi Kawai
Yaeko Nishizawa
Kazuko Ono
Kiyomi Abe
Yuichi Ishikawa
Hidemitsu Nakamura
Hiroaki Ichikawa
Shigeo Nishimura
Tsutomu Shimizu
Seiichi Toki
机构
[1] National Institute of Agrobiological Sciences,Department of Plant Biotechnology
[2] Tsukuba University,Graduate School of Life and Environmental Sciences
[3] Kumiai Chemical Industry Co.,Life Science Research Institute
[4] Tohoku University,Graduate School of Life Sciences
来源
Molecular Breeding | 2005年 / 16卷
关键词
Acetolactate synthase; Herbicide resistance; Promoter; Rice; Selection marker; Transgenic;
D O I
暂无
中图分类号
学科分类号
摘要
The proper use of a marker gene in a transformation process is critical for the production of transgenic plants. However, consumer concerns and regulatory requirements raise an objection to the presence of exogenous DNA in transgenic plants, especially antibiotic-resistant genes and promoters derived from viruses. One approach to overcome this problem is the elimination of marker genes from the plant genome by using several site-specific recombination systems. We propose an alternative method to solve this problem using a marker gene exclusively derived from the host plant DNA. We cloned a genomic DNA fragment containing regulatory and coding sequences of acetolactate synthase (ALS) gene from rice, and mutagenized the ALS gene into a herbicide-resistant form. After transfer of this construct to the rice genome, transgenic plants were efficiently selected with a herbicide, bispyribac-sodium salt, which inhibits the activity of wild type ALS. We also analyzed the regulatory feature of the rice ALS gene promoter with the gusA reporter gene and revealed that GUS expression was observed constitutively in aerial parts of rice seedlings and root tips. The marker system consisted exclusively of host plant DNA and enabled efficient selection in a monocot crop plant, rice. The selection system can potentially be applied to generate transgenic plants of other crop species and can be expected to be publicly acceptable.
引用
收藏
页码:313 / 320
页数:7
相关论文
共 42 条
  • [21] Mutagenesis Analysis of Acetolactate Synthase (ALS) Genes from Vitis vinifera for Use as a Selectable Marker to Facilitate Precision Breeding of Grapevine
    Li, Zhijian
    Chi Nguyen
    Dean, Deborah
    Grant, Trudi
    Creech, Matthew
    Das, Bishu
    Gray, Dennis
    HORTSCIENCE, 2015, 50 (09) : S146 - S146
  • [22] A mutant dihydrofolate reductase-thymidylate synthase from Leishmania major as a selectable marker in transfection experiments
    Arrebola, R
    Olmo, A
    Camacho, A
    RuizPerez, LM
    GonzalezPacanowska, D
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 79 (01) : 129 - 133
  • [23] Current topics of plant genetic transformation technology: Utilization of a selectable marker gene (mALS) from plant for genetic transformation
    Toki, Seiichi
    Shimizu, Tsutomu
    JOURNAL OF PESTICIDE SCIENCE, 2010, 35 (02) : 151 - 151
  • [24] An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice
    Erika Toda
    Narumi Koiso
    Arika Takebayashi
    Masako Ichikawa
    Takatoshi Kiba
    Keishi Osakabe
    Yuriko Osakabe
    Hitoshi Sakakibara
    Norio Kato
    Takashi Okamoto
    Nature Plants, 2019, 5 : 363 - 368
  • [25] An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice
    Toda, Erika
    Koiso, Narumi
    Takebayashi, Arika
    Ichikawa, Masako
    Kiba, Takatoshi
    Osakabe, Keishi
    Osakabe, Yuriko
    Sakakibara, Hitoshi
    Kato, Norio
    Okamoto, Takashi
    NATURE PLANTS, 2019, 5 (04) : 363 - 368
  • [26] Genetic transformation with the sull gene; A highly efficient selectable marker for Solanum tuberosum L cv 'Russet Burbank'
    Wallis, JG
    Dziewanowska, K
    Guerra, DJ
    MOLECULAR BREEDING, 1996, 2 (03) : 283 - 290
  • [27] The Bacterial Phytoene Desaturase-Encoding Gene (CRTI) is an Efficient Selectable Marker for the Genetic Transformation of Eukaryotic Microalgae
    Molina-Marquez, Ana
    Vila, Marta
    Vigara, Javier
    Borrero, Ana
    Leon, Rosa
    METABOLITES, 2019, 9 (03):
  • [28] A novel mutant acetolactate synthase gene from rice cells, which confers resistance to ALS-inhibiting herbicides
    Kawai, Kiyoshi
    Kaku, Koichiro
    Izawa, Norihiko
    Shimizu, Tsutomu
    Fukuda, Atsunori
    Tanaka, Yoshiyuki
    JOURNAL OF PESTICIDE SCIENCE, 2007, 32 (02) : 89 - 98
  • [29] A novel mutant acetolactate synthase gene from rice cells, which confers resistance to ALS-inhibiting herbicides
    Kawai, Kiyoshi
    Kaku, Koichiro
    Izawa, Norihiko
    Sinmizu, Tsutomu
    Fukuda, Atsunori
    Tanaka, Yoshiyuki
    JOURNAL OF PESTICIDE SCIENCE, 2008, 33 (03) : 278 - 280
  • [30] Agrobacterium-mediated co-transformation of rice using two selectable marker genes derived from rice genome components
    Wakasa, Yuhya
    Ozawa, Kenjirou
    Takaiwa, Fumio
    PLANT CELL REPORTS, 2012, 31 (11) : 2075 - 2084