Ultrastructural changes in pancreatic beta cells treated with NGF and dbcAMP

被引：0

作者：

González del Pliego M. ^{[1
]}

Aguirre-Benítez E. ^{[1
]}

Del Carmen Sánchez-Soto M. ^{[2
]}

Larrieta M. ^{[2
]}

Velázquez-Carranza A. ^{[1
]}

Vidaltamayo R. ^{[2
]}

Meza-Mora M. ^{[1
]}

Zepeda-Rodríguez A. ^{[4
]}

Hernández-Falcón J. ^{[3
]}

Hiriart M. ^{[2
]}

机构：

[1] Facultad de Medicina, Depto. de Embriología, Ciudad Universitaria, México D.F.

[2] Instituto de Fisiología Celular, Depto. Biofísica, Ciudad Universitaria, México D.F.

[3] Facultad de Medicina, Depto. de Fisiología, Ciudad Universitaria, México D.F.

[4] Facultad de Medicina, Depto. de Biología Celular y Tisular, Ciudad Universitaria, México D.F.

来源：

Cell and Tissue Research | 2001年 / 305卷 / 3期

关键词：

Cell plasticity; GABA; Growth factor; Insulin; Pancreatic islet cells; Rat (Wistar);

D O I：

10.1007/s004410100411

中图分类号：

学科分类号：

摘要：

Nerve growth factor (NGF) induces morphological and physiological changes in cultured pancreatic β-cells, including the extension of neurite-like processes. This latter effect is potentiated by dibutyryl cAMP (dbcAMP). β-cells cultured under these conditions maintain their immunoreactivity to insulin and gamma-amino-butyric acid (GABA). NGF, dbcAMP, and high glucose concentrations also increase the expression of the GABA-synthesizing enzyme glutamic acid decarboxylase-65 in cultured β-cells. The aim of this work was to study the effect of NGF alone or in combination with dbcAMP on pancreatic β-cell ultrastructural morphology, after 10 days in culture. We used light microscopy, scanning electron microscopy, and transmission electron microscopy to analyze the modifications in cell surface and neurite-like projections. Morphometric analysis showed that NGF and/or dbcAMP treatment substantially increased the insulin and GABA content in granules and rough endoplasmic reticulum. Given that pancreatic β-cells express NGF receptors and that NGF is synthesized and secreted by β-cells, these results further suggest that NGF could have trophic actions on pancreatic hormone synthesis and/or storage.

引用

页码：365 / 378

页数：13

共 40 条

[1]

Baekkeskov S., Aanstoot H.J., Christagau S., Reetz A., Solimena M., Cascalho M., Folli F., Richter-Olesen H., De Camilli P., Identification of a 64 kDa autoantigen in insulin-dependent diabetes as the GABA-synthesising enzyme glutamic acid decarboxylase, Nature, 347, pp. 151-156, (1990)

[2]

Bani-Sacchi T., Bani D., New views on the identification of the various cell types in the pancreatic islets of the rat, Acta Anat, 122, pp. 1-17, (1985)

[3]

Baskin D.G., Gorray K.C., Fujimoto W.Y., Immunocytochemical identification of cells containing insulin, glucagon, somatostatin, and pancreatic polypeptide in the islets of Langerhans of the guinea pig pancreas with light and electron microscopy, Anat Rec, 208, pp. 567-578, (1984)

[4]

Bendayan M., Ultrastructural localization of insulin and C-peptide antigenic sites in rat pancreatic β cell obtained by applying the quantitative high-resolution protein A-gold approach, Am J Anat, 185, pp. 205-216, (1989)

[5]

Christgau S., Schierbeck H., Aanstoot H.J., Aagaard L., Begley K., Kofod H., Hejnaes K., Baekkeskov S., Pancreatic beta cells express two autoantigenic forms of glutamic decarboxylase, a 65-kDa hydrophilic form and a 64-kDa amphiphilic which can be both membrane-bound and soluble, J Biol Chem, 266, pp. 21257-21264, (1991)

[6]

Drubin D., Kobayashi S., Kellogg D., Kirschner M., Regulation of microtubule protein levels during cellular morphogenesis in nerve growth factor-treated PC12 cells, J Cell Biol, 106, pp. 1583-1591, (1988)

[7]

Elayat A.A., El-Naggar M.M., Tahir M., An immunocytochemical and morphometric study of the rat pancreatic islets, J Anat, 186, pp. 629-637, (1995)

[8]

Feria-Velasco A., Castillo-Medina S., Verdugo-Diaz L., Castellanos E., Orozco-Suarez S., Sanchez-Gomez C., Drucker-Colin R., Neuronal of chromaffin cells in vitro, induced by extremely low frequency magnetic fields or nerve growth factor: A histological and ultrastructuraal comparative study, J Neurosci Res, 53, pp. 569-582, (1998)

[9]

Frodin M., Sekine N., Roche E., Filloux C., Prentki M., Wollheim C.B., Van Obberghen E., Glucose, other secretagogues, and nerve growth factor stimulate mitogen-activated protein kinase in the insulin-secreting beta-cell line, INS-1, J Biol Chem, 270, pp. 7882-7889, (1995)

[10]

Garry D.J., Coulter H.D., McIntee T.J., Wu J.Y., Sorenson R.L., Immunoreactive GABA transaminase within the pancreatic islet is localized in mitochondria of the β-cell, J Histochem Cytochem, 35, pp. 831-836, (1987)

← 1 2 3 4 →