A nanomechanical interface to rapid single-molecule interactions

被引：0
作者：
Mingdong Dong
Ozgur Sahin
机构：
[1] The Rowland Institute at Harvard,
[2] Harvard University,undefined
[3] Present addresses: The Interdisciplinary Nanoscience Center (iNANO),undefined
[4] Centre for DNA Nanotechnology (CDNA),undefined
[5] Aarhus University,undefined
[6] Aarhus C DK-8000,undefined
[7] Denmark (M.D.); Wyss Institute for Biologically Inspired Engineering,undefined
[8] Harvard University,undefined
[9] Boston,undefined
[10] Massachusetts 02115,undefined
[11] USA (O.S.).,undefined
来源：
Nature Communications | / 2卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Single-molecule techniques provide opportunities for molecularly precise imaging, manipulation, assembly and biophysical studies. Owing to the kinetics of bond rupture processes, rapid single-molecule measurements can reveal novel bond rupture mechanisms, probe single-molecule events with short lifetimes and enhance the interaction forces supplied by single molecules. Rapid measurements will also increase throughput necessary for technological use of single-molecule techniques. Here we report a nanomechanical sensor that allows single-molecule force spectroscopy on the previously unexplored microsecond timescale. We probed bond lifetimes around 5 μs and observed significant enhancements in molecular interaction forces. Our loading-rate-dependent measurements provide experimental evidence for an additional energy barrier in the biotin–streptavidin complex. We also demonstrate quantitative mapping of rapid single-molecule interactions with high spatial resolution. This nanomechanical interface may allow studies of molecular processes with short lifetimes and development of novel biological imaging, single-molecule manipulation and assembly technologies.
引用
收藏
相关论文
共 109 条
[1]  
Zhang SG(2003)Fabrication of novel biomaterials through molecular self-assembly Nat. Biotechnol. 21 1171-1178
[2]  
Ke YG(2008)Self-assembled water-soluble nucleic acid probe tiles for label-free RNA hybridization assays Science 319 180-183
[3]  
Lindsay S(2006)Folding DNA to create nanoscale shapes and patterns Nature 440 297-302
[4]  
Chang Y(2006)Detection and localization of single molecular recognition events using atomic force microscopy Nat. Methods 3 347-355
[5]  
Liu Y(2004)Single-molecule recognition imaging-microscopy Proc. Natl Acad. Sci. USA 101 12503-12507
[6]  
Yan H(2008)Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy Nat. Methods 5 491-505
[7]  
Rothemund PWK(1994)Adhesion forces between individual ligand-receptor pairs Science 264 415-417
[8]  
Hinterdorfer P(2005)Nanoscale mapping and functional analysis of individual adhesins on living bacteria Nat. Methods 2 515-520
[9]  
Dufrene YF(2009)Stretching single talin rod molecules activates vinculin binding Science 323 638-641
[10]  
Stroh C(2008)Single-molecule cut-and-paste surface assembly Science 319 594-596
12345678910