Microvesicles shed from microglia activated by the P2X7-p38 pathway are involved in neuropathic pain induced by spinal nerve ligation in rats

被引：0
作者：
Jian Li
Xiangnan Li
Xin Jiang
Mei Yang
Rui Yang
Geoffrey Burnstock
Zhenghua Xiang
Hongbin Yuan
机构：
[1] Second Military Medical University,Department of Anesthesiology, Changzheng Hospital
[2] The Third People’s Hospital of Yancheng,Department of Anesthesiology
[3] University College Medical School,Autonomic Neuroscience Centre
[4] The University of Melbourne,Department of Pharmacology and Therapeutics
[5] Second Military Medical University,Department of Neurobiology, MOE Key Laboratory of Molecular Neurobiology, Ministry of Education
来源：
Purinergic Signalling | 2017年 / 13卷
关键词：
Microvesicles; Microglia; Neuropathic pain; Spinal nerve ligation, P2X7;
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学科分类号：
摘要：
Microglia are critical in the pathogenesis of neuropathic pain. In this study, we investigated the role of microvesicles (MVs) in neuropathic pain induced by spinal nerve ligation (SNL) in rats. First, we found that MVs shed from microglia were increased in the cerebrospinal fluid and dorsal horn of the spinal cord after SNL. Next, MVs significantly reduced paw withdrawal threshold (PWT) and paw withdrawal latency (PWL). In addition, the P2X7-p38 pathway was related to the bleb of MVs after SNL. Interleukin (IL)-1β was found to be significantly upregulated in the package of MVs, and PWT and PWL increased following inhibition with shRNA-IL-1β. Finally, the amplitude and frequency of spontaneous excitatory postsynaptic currents increased following stimulation with MVs. Our results indicate that the P2X7-p38 pathway is closely correlated with the shedding of MVs from microglia in neuropathic pain, and MVs had a significant effect on neuropathic pain by participating in the interaction between microglia and neurons.
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页码：13 / 26
页数：13
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