Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes

被引:0
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作者
Justin York
David Spetzler
Tassilo Hornung
Robert Ishmukhametov
James Martin
Wayne D. Frasch
机构
[1] Arizona State University,School of Life Sciences
来源
Journal of Bioenergetics and Biomembranes | 2007年 / 39卷
关键词
F; -ATPase; Gold nanorods; Plasmon resonance; Molecular motors; Single molecule microscopy;
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摘要
The abundance of E. coli F1-ATPase molecules observed to rotate using gold nanorods attached to the γ-subunit was quantitated. Individual F1 molecules were determined to be rotating based upon time dependent fluctuations of red and green light scattered from the nanorods when viewed through a polarizing filter. The average number of F1 molecules observed to rotate in the presence of GTP, ATP, and without nucleotide was ∼50, ∼25, and ∼4% respectively. In some experiments, the fraction of molecules observed to rotate in the presence of GTP was as high as 65%. These data indicate that rotational measurements made using gold nanorods provide information of the F1-ATPase mechanism that is representative of the characteristics of the enzyme population as a whole.
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页码:435 / 439
页数:4
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