Conjugation of imipenem to silver nanoparticles for enhancement of its antibacterial activity against multidrug-resistant isolates of Pseudomonas aeruginosa

被引：0
作者：
Mahsa Shahbandeh
Anoosh Eghdami
Mehrdad Moosazadeh Moghaddam
Mohammadreza Jalali Nadoushan
Ali Salimi
Mahdi Fasihi-Ramandi
Saber Mohammadi
Mehrnaz Mirzaei
Reza Mirnejad
机构：
[1] Islamic Azad University,Young Researchers and Elite Club, Saveh Branch
[2] Islamic Azad University,Department of Biochemistry, Medical Faculty Member, Saveh Branch
[3] Baqiyatallah University of Medical Sciences,Applied Biotechnology Research Center
[4] Shahed University,Department of Pathology
[5] Baqiyatallah University of Medical Sciences,Nanobiotechnology Research Center
[6] Baqiyatallah University of Medical Sciences,Molecular Biology Research Center, Systems Biology and Poisonings Institute
[7] Islamic Azad University,Young Researchers and Elite Club
[8] Islamic Azad University,Department of Microbiology, Tehran Medical Sciences Branch
来源：
Journal of Biosciences | 2021年 / 46卷
关键词：
multidrug resistance; imipenem; silver nanoparticle; conjugation; antibacterial;
D O I：
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摘要：
Due to the broad-spectrum of antibiotic resistance, herein we investigated the possibility of using imipenem-conjugated silver nanoparticles (IMP-AgNPs) against multidrug-resistant isolates of Pseudomonas aeruginosa. For this purpose, 200 clinical isolates were tested against different antibiotics to determine the antimicrobial susceptibility. To identify blaVIM and blaIMP resistance genes, PCR was used. The synthesized AgNPs and conjugants were characterized using UV-vis spectroscopy, XRD, SEM, TEM, DLS, and FTIR. The stability, drug release kinetics, cytotoxicity, hemolytic and apoptotic effects of NPs were also investigated. MIC of the imipenem, AgNPs, and conjugants were evaluated versus P. aeruginosa isolates. Finally, the effects of the IMP-AgNPs to heal burn wounds in rats was evaluated. According to the results, about 68% of isolates showed resistance to imipenem (MIC ≥ 64 μg/ml to ≥ 512 μg/ml). Analytical results verified the synthesis of AgNPs and IMP-AgNPs. A Dose-dependent decrease happened in terms of the MIC values of IMP-AgNPs were also affected by the existence of resistant genes. Low cytotoxic was observed regarding AgNPs which lead to apoptosis. The histopathological results showed a considerable epithelization in treated groups with IMP-AgNPs. Accordingly, IMP-AgNPs can be considered as a powerful antibacterial agent to treat the infections caused by multidrug-resistant P. aeruginosa.
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