A rapid HPLC method for the determination of carboxylic acids in human urine using a monolithic column

被引：0
作者：
I. Šperlingová
L. Dabrowská
V. Stránský
M. Tichý
机构：
[1] National Institute of Public Health,
来源：
Analytical and Bioanalytical Chemistry | 2004年 / 378卷
关键词：
Carboxylic acids; Monolithic column; Human urine;
D O I：
暂无
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学科分类号：
摘要：
A rapid HPLC method for the determination of carboxylic acids in urine samples using a Chromolith Performance RP/18e 100/4.6 with Chromolith Guard Cartridge RP/18e 10/4.6 (Merck KgaA, Darmstadt, Germany) was developed. The method facilitates the simultaneous determination of aromatic hydrocarbon metabolites mandelic acid (MA) and phenylglyoxylic acid (PGA) from styrene and ethylbenzene, hippuric acid (HA) from toluene and 2-, 3-, 4-methylhippuric acids (MHA) from xylene. 3-Hydroxybenzoic acid (3-HBA) was used as internal standard. A chromatographic run is completed within less than 5 min for styrene, ethylbenzene and toluene metabolites, and within 10 min for xylene metabolites. The detection limits are 9 mg L−1 urine for MA, 1.25 mg L−1 urine for PGA, 4.9 mg L−1 urine for HA, 22 mg L−1 urine for 2-MHA, and 18.5 mg L−1 urine for 3-MHA.
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页码：536 / 543
页数：7
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