Over-expression of ArabidopsisFT gene reduces juvenile phase and induces early flowering in ornamental gentian plants

被引：0
作者：
Takashi Nakatsuka
Yoshiko Abe
Yuko Kakizaki
Akiko Kubota
Norimoto Shimada
Masahiro Nishihara
机构：
[1] Iwate Biotechnology Research Center,
来源：
Euphytica | 2009年 / 168卷
关键词：
Early flowering; FT; Gentian; In vitro flowering; Transgenic plant;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Ornamental gentian plants are perennial and have a juvenile period of over 1 year before flowering. We transformed gentian plants with a construct comprising the ArabidopsisFLOWERING LOCUS T (FT) gene (encoding a major component protein of the flowering hormone ‘florigen’) under the control of the rolC promoter from Agrobacterium rhizogenes, which is known to induce vascular-specific expression. The resultant rolCpro-FT transgenic gentian plants showed early flowering in vitro and the earliest line formed floral buds within 4 months after transformation. Regeneration experiments from leaf explants of these rolCpro-FT transgenic plants also confirmed the early flowering phenotype. After acclimatization, these transgenic plants showed normal floral development in a closed greenhouse. There is no effective method to induce early flowering by cultivation management in gentian, therefore these lines might be very useful as annual early season cultivars.
引用
收藏
页码：113 / 119
页数：6
相关论文
共 127 条
[1]  
Abe M(2005)FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex Science 309 1052-1056
[2]  
Kobayashi Y(2006)/ Science 312 1040-1043
[3]  
Yamamoto S(2007) regulatory module controls timing of flowering and seasonal growth cessation in trees Science 316 1030-1033
[4]  
Daimon Y(2005)FT protein movement contributes to long-distance signaling in floral induction of Transgenic Res 14 703-712
[5]  
Yamaguchi A(2007)Ectopic expression of an Mol Genet Genomics 278 95-104
[6]  
Ikeda Y(1993) homolog from citrus confers an early flowering phenotype on trifoliate orange ( Plant Mol Biol 21 17-26
[7]  
Ichinoki H(1996) L. Raf.) Plant Cell Rep 15 578-581
[8]  
Notaguchi M(1999)Structure and allele-specific expression variation of novel alpha/beta hydrolase fold proteins in gentian plants Science 286 1962-1965
[9]  
Goto K(1999)Transgene copy number can be positively or negatively associated with transgene expression Science 286 1960-1962
[10]  
Araki T(2002)Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Plant Cell Physiol 43 1096-1105
12345678910