Potato leafroll virus detection by RT-PCR in field-collected aphids

被引：0
作者：
Rudra P. Singh
J. Kurz
G. Boiteau
Lynn M. Moore
机构：
[1] Agriculture and Agri-Food Canada,Potato Research Centre
[2] New Brunswick Department of Agriculture and Rural Development,undefined
来源：
American Potato Journal | 1997年 / 74卷
关键词：
RT-PCR; virus detection; epidemiology; virus transmission; virus spread;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A reverse transcription polymerase chain reaction (RT-PCR) assay was applied to analyze green peach aphids (Myzus persicae Sulzer) collected from yellow-pan traps between 1988 and 1994, from New Brunswick commercial potato fields, for potato leafroll virus (PLRV). The RT-PCR technique was also used to assess the PLRV content ofMyzus persicae Sulzer,Aphis nasturtii Kaltenbach, andMacrosiphum euphorbiae Thomas caught in yellow-pan traps from selected fields in 1995. RT-PCR bands specific for PLRV were detected in all three species, indicating that they all can acquire the virus. The usefulness of the RT-PCR procedure in determining the PLRV content of aphid catches and thus assisting in disease management recommendations is discussed.
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页码：305 / 313
页数：8
相关论文
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