Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification–based assay for GBS screening during pregnancy

被引：0
作者：
Mireille Tittel-Elmer
Begoña Martinez de Tejada
Gesuele Renzi
Jacques Schrenzel
机构：
[1] Geneva University Hospitals,Bacteriology Laboratory, Division of Laboratory Medicine, Department of Diagnostics
[2] Geneva University Hospitals,Obstetrics Division, Departement of Pediatrics, Gynecology and Obstetrics
[3] University of Geneva,Faculty of Medicine
[4] Geneva University Hospitals,Genomic Research Laboratory, Division of Infectious Diseases, Department of Medicine
来源：
European Journal of Clinical Microbiology & Infectious Diseases | 2023年 / 42卷
关键词：
Group B streptococci; LAMP assay; Intrapartum prophylaxis; Early onset neonatal sepsis; Screening; Pregnancy;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Timely and accurate detection of Group B Streptococcus (GBS) carriage in pregnant women allows for targeted peripartum prophylaxis. Replacing culture-based screening by molecular biology assays enables faster results obtention, better targeted antibiotic prophylaxis, and reduces the laboratory workload. Here, we present a comparative analysis between a Loop Mediated Isothermal Amplification assay (HiberGene GBS kit) and culture (gold-standard). The HiberGene GBS kit showed a sensitivity of 97.9% and a specificity of 96.8% compared with culture. The limit of detection was estimated at 103 cfu/ml and results were obtained within 30 min. HiberGene GBS assay can be used for peripartum GBS screening and targeted antibiotic prophylaxis provided sample processing can be swiftly performed around the clock.
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页码：217 / 219
页数：2
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