Chemicobiological Insight into Anti-phytopathogenic Properties of Rhizospheric Serratia plymuthica R51

被引：6

作者：

John C.J. ^{[1
]}

Radhakrishnan E.K. ^{[1
]}

机构：

[1] School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, 686 560, Kerala

来源：

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2018年 / 88卷 / 4期

关键词：

Antifungal mechanisms; Rhizobacteria; Serratia plymuthica;

D O I：

10.1007/s40011-017-0909-1

中图分类号：

学科分类号：

摘要：

Interest in biocontrol of plant pathogens has increased in recent years due to agricultural trend towards greater sustainability and public concern over the use of hazardous pesticides. In the present study, rhizobacteria isolated from Curcuma amada were screened for their antifungal activity against phytopathogens. Among these, the isolate R51 was found to have promising activity against selected phytopathogens in dual culture. The antifungal compound containing fraction was purified by column chromatography and was identified by ultra violet spectrum (UV), infra-red spectrum (FT-IR) and mass spectroscopic (LC–MS) analyses as pyrrolnitrin. By scanning electron microscopic (SEM) analysis, the isolate R51 was found to cause vacuolation, swelling, distortion and lysis of mycelia of the pathogenic fungi. R51 was identified as Serratia plymuthica and was found to provide effective protection to ginger rhizome from soft-rot caused by Pythium myriotylum. Hence, the chemicobiological basis of antifungal activity of Serratia plymuthica can be due to pyrrolnitrin [3-chloro-4-(2′-nitro-3′-chlorophenyl) pyrrole] production and is having significant agricultural applications. © 2017, The National Academy of Sciences, India.

引用

页码：1629 / 1635

页数：6

共 30 条

[1]

Ahemad M., Kibret M., Mechanisms and applications of plant growth promoting rhizobacteria: current perspective, J King Saud Univ Sci, 26, pp. 1-20, (2014)

[2]

Vleesschauwer D., Using Serratia plymuthica to control fungal pathogens of plants, CAB Rev Perspect Agric Vet Sci Nutr Nat Resour, 2, (2007)

[3]

Perneel M., Heyrman J., Adiobo A., De Maeyer K., Raaijmakers J.M., De Vos P., Hofte M., Characterization of CMR5c and CMR12a, novel fluorescent Pseudomonas strains from the cocoyam rhizosphere with biocontrol activity, J Appl Microbiol, 103, pp. 1007-1020, (2007)

[4]

Raaijmakers J.M., Vlami M., de Souza J.T., Antibiotic production by bacterial biocontrol agents, Antonie Van Leeuwenhoek, 81, pp. 537-547, (2002)

[5]

Souza J.T., Raaijmakers J.M., Polymorphisms within the prnD and pltC genes from pyrrolnitrin and pyoluteorin-producing Pseudomonas and Burkholderia spp, FEMS Microbiol Ecol, 43, pp. 21-34, (2003)

[6]

Deng P., Wang X., Baird S.M., Lu S.E., Complete genome of Pseudomonas chlororaphis strain UFB2, a soil bacterium with antibacterial activity against bacterial canker pathogen of tomato, Stand Genomic Sci, 10, (2015)

[7]

Zivkovic S., Stojanovic S., Ivanovic Z., Gavrilovic V., Popovic T., Balaz J., Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides, Arch Biol Sci, 62, pp. 611-623, (2010)

[8]

Chun J., Goodfellow M., A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences, Int J Syst Bacteriol, 45, pp. 240-245, (1995)

[9]

Zhang Z., Schwartz S., Wagner L., Miller W., A greedy algorithm for aligning DNA sequences, J Comput Biol, 7, pp. 203-214, (2000)

[10]

Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol Biol Evol, 28, pp. 2731-2739, (2011)

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