Effects of a Brain-Engraftable Microglial Cell Line Expressing Anti-Prion scFv Antibodies on Survival Times of Mice Infected with Scrapie Prions

被引：0
作者：
Koji Fujita
Yoshitaka Yamaguchi
Tsuyoshi Mori
Naomi Muramatsu
Takahito Miyamoto
Masashi Yano
Hironori Miyata
Akira Ootsuyama
Makoto Sawada
Haruo Matsuda
Ryuji Kaji
Suehiro Sakaguchi
机构：
[1] The University of Tokushima,Division of Molecular Neurobiology, The Institute for Enzyme Research
[2] The University of Tokushima Graduate School,Department of Clinical Neuroscience
[3] University of Occupational and Environmental Health,Animal Research Center
[4] University of Occupational and Environmental Health,Department of Radiation Biology and Health, School of Medicine
[5] Nagoya University,Department of Brain Functions, Division of Stress Adaptation and Protection, Research Institute of Environmental Medicine
[6] Hiroshima University,Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Laboratory of Immunology
来源：
Cellular and Molecular Neurobiology | 2011年 / 31卷
关键词：
Prion; Immunotherapy; ScFv; Ex vivo gene transfer; Brain-engraftable cells;
D O I：
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学科分类号：
摘要：
We first verified that a single chain Fv fragment against prion protein (anti-PrP scFv) was secreted by HEK293T cells and prevented prion replication in infected cells. We then stably expressed anti-PrP scFv in brain-engraftable murine microglial cells and intracerebrally injected these cells into mice before or after infection with prions. Interestingly, the injection before or at an early time point after infection attenuated the infection marginally but significantly prolonged survival times of the mice. These suggest that the ex vivo gene transfer of anti-PrP scFvs using brain-engraftable cells could be a possible immunotherapeutic approach against prion diseases.
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