Pharmacological investigation of voltage-dependent Ca2+ channels in human ejaculatory sperm in vitro

被引：4

作者：

Li L. ^{[1
]}

Liu J. ^{[1
]}

Li J. ^{[1
]}

Ye Z. ^{[1
]}

机构：

[1] Department of Urology, Tongji Hospital, Huazhong University of Science and Technology

来源：

Journal of Huazhong University of Science and Technology | 2006年 / 26卷 / 5期

关键词：

Calcium channel blocker; Human sperm motility; In vitro; Voltage-dependent calcium channels;

D O I：

10.1007/s11596-006-0534-3

中图分类号：

学科分类号：

摘要：

The types of the voltage-dependent calcium channels (VDCCs) in human ejaculatory sperm and the effects of calcium channel blocker (CCB) on human sperm motility parameters in vitro were investigated. The human sperm motility parameters in vitro in response to the pharmacological agents nifedipine (NIF, inhibitor of L-type VDCC) and ω-contoxin (GVIA, inhibitor of N-type VDCC) were compared and analyzed statistically. The results showed that NIF (1, 5, 10 μmol/L) could not only significantly affect human sperm's shape but also spermatozoa motility after incubated at least 10 min in vitro (P<0.001). GVIA (0.1, 0.5 and 1 μmol/L) could just only significantly affect human sperm's progressive motility (a %+b %) after incubated for 20 min in vitro (P<0.01), but they both could not significantly affect spermic abnormality rate. It is suggested that L-type VDCC, non L-type VDCCs and isoform of L-type VDCC exist in the cell membrane of human sperm solely or together, and they participate in the spermic physiological processes especially the spermic motility. © 2006 Huazhong University of Science and Technology.

引用

页码：607 / 609

页数：2

共 19 条

[1]

Breitbart H., Intracellular calcium regulation in sperm capacitation and acrosomal reaction, Mol Cell Endocrinol, 187, pp. 139-144, (2002)

[2]

Allamaneni S.S., Agarwal A., Rama S., Et al., Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa, Asian Androl, 7, pp. 86-92, (2005)

[3]

Hauser R., Godfrey-Bailey L., Chen Z., Does the potential for selection bias in semen quality studies depend on study design? Experience from a study conducted within an infertility clinic, Hum Reprod, 20, pp. 2579-2583, (2005)

[4]

Kirkman-Brown J.C., Barratt C.L., Publicover S.J., Nifedipine reveals the existence of two discrete components of the progesterone-induced [Ca<sup>2+</sup>]i transient in human spermatozoa, Dev Biol, 259, pp. 71-82, (2003)

[5]

Arikkath J., Campbell K.P., Auxiliary subunits: Essential components of the voltage-gated calcium channel complex, Curr Opin Neurobiol, 13, pp. 298-307, (2003)

[6]

Kang M.G., Campbell K.P., Gamma subunit of voltage-activated calcium channels, Biol Chem, 13, pp. 21315-21318, (2003)

[7]

Catterall W.A., Structure and regulation of voltage-gated Ca<sup>2+</sup> channels, Annu Rev Cell Dev Biol, 16, pp. 521-555, (2000)

[8]

Catterall W.A., Striessnig J., Snutch T.P., Et al., Compendium of voltage-gated ion channels: Calcium channels, Pharmacol Rev, 55, pp. 579-581, (2003)

[9]

Koshimizu T.A., Van Goor F., Tomic M., Et al., Characterization of calcium signaling by purinergic receptor-channels expressed in excitable cells, Mol Pharmacol, 58, pp. 936-981, (2000)

[10]

Darszon A., Wood C.D., Beltran C., Et al., Measuring ion fluxes in sperm, Methods Cell Biol, 74, pp. 545-576, (2004)

← 1 2 →