Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan

被引：17

作者：

Belmans, Niels ^{[1
,2
]}

Gilles, Liese ^{[1
]}

Vermeesen, Randy ^{[2
]}

Virag, Piroska ^{[3
]}

Hedesiu, Mihaela ^{[4
]}

Salmon, Benjamin ^{[5
,6
]}

Baatout, Sarah ^{[2
]}

Lucas, Stephane ^{[7
]}

Lambrichts, Ivo ^{[1
]}

Jacobs, Reinhilde ^{[8
,9
,10
]}

Moreels, Marjan ^{[2
]}

Oenning, A. C. ^{[5
,6
]}

Chaussain, C. ^{[5
,6
]}

Lefevre, C. ^{[5
,6
]}

Baciut, M. ^{[4
]}

Marcu, M. ^{[4
]}

Almasan, O. ^{[4
]}

Roman, R. ^{[4
]}

Barbur, I. ^{[4
]}

Dinu, C. ^{[4
]}

Rotaru, H. ^{[4
]}

Hurubeanu, L. ^{[4
]}

Istouan, V. ^{[4
]}

Lucaciu, O. ^{[4
]}

Leucuta, D. ^{[4
]}

Crisan, B. ^{[4
]}

Bogdan, L. ^{[4
]}

Candea, C. ^{[4
]}

Bran, S. ^{[4
]}

Baciut, G. ^{[4
]}

Bosmans, H. ^{[8
,9
]}

Bogaerts, R. ^{[8
,9
]}

Politis, C. ^{[8
,9
]}

Stratis, A. ^{[8
,9
]}

Pauwels, R. ^{[8
,9
]}

Vasconcelos, K. de F. ^{[8
,9
]}

Nicolielo, L. ^{[8
,9
]}

Zhang, G. ^{[8
,9
]}

Tijskens, E. ^{[8
,9
]}

Vranckx, M. ^{[8
,9
]}

Ockerman, A. ^{[8
,9
]}

Claerhout, E. ^{[8
,9
]}

Embrechts, E. ^{[8
,9
]}

机构：

[1] Hasselt Univ, Morphol Grp, Biomed Res Inst, Agoralaan Bldg C, Diepenbeek, Belgium

[2] SCK CEN, Belgian Nucl Res Ctr, Radiobiol Unit, Mol, Belgium

[3] Inst Oncol Prof Dr Ion Chiricuta, Cluj Napoca, Romania

[4] Iuliu Hatieganu Univ Med & Pharm, Dept Oral & Maxillofacial Radiol, Cluj Napoca, Romania

[5] Paris Descartes Univ, Sorbonne Paris Cite, EA 2496, Orofacial Pathol Imaging & Biotherapies Lab, Paris, France

[6] Bretonneau Hosp, AP HP, HUPNVS, Dept Dent Med, Paris, France

[7] Univ Namur, Namur Res Inst Life Sci, Namur, Belgium

[8] Katholieke Univ Leuven, Dept Imaging & Pathol, OMFS IMPATH Res Grp, Kapucijnenvoer 7, Leuven, Belgium

[9] Univ Hosp, Oral & Maxillofacial Surg, Dentomaxillofacial Imaging Ctr, Kapucijnenvoer 7, Leuven, Belgium

[10] Karolinska Inst, Dept Dent Med, Huddinge, Sweden

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

BUCCAL MUCOSA CELLS; CONE-BEAM CT; GAMMA-H2AX FOCI; CANCER-RISK; COMPUTED-TOMOGRAPHY; IONIZING-RADIATION; ADAPTIVE RESPONSE; SUBSEQUENT RISK; NUCLEOTIDE POOL; BRAIN-TUMOR;

D O I：

10.1038/s41598-020-58746-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Assessing the possible biological effects of exposure to low doses of ionizing radiation (IR) is one of the prime challenges in radiation protection, especially in medical imaging. Today, radiobiological data on cone beam CT (CBCT) related biological effects are scarce. In children and adults, the induction of DNA double strand breaks (DSBs) in buccal mucosa cells and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and antioxidant capacity in saliva samples after CBCT examination were examined. No DNA DSBs induction was observed in children nor adults. In children only, an increase in 8-oxo-dG levels was observed 30 minutes after CBCT. At the same time an increase in antioxidant capacity was observed in children, whereas a decrease was observed in adults. Our data indicate that children and adults react differently to IR doses associated with CBCT. Fully understanding these differences could lead to an optimal use of CBCT in different age categories as well as improved radiation protection guidelines.

引用

页数：13

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