Fast Analysis of Total Polyphenol Content and Antioxidant Activity in Wines and Oenological Tannins Using a Flow Injection System with Tandem Diode Array and Electrochemical Detections

被引：0
作者：
Arianna Ricci
Nemanja Teslic
Violeta-Ivanova Petropolus
Giuseppina Paola Parpinello
Andrea Versari
机构：
[1] University of Bologna,Department of Agricultural and Food Sciences
[2] University of Novi Sad,Institute of Food Technology
[3] University “Goce Delčev”,Faculty of Agriculture
来源：
Food Analytical Methods | 2019年 / 12卷
关键词：
Total polyphenol content; Antioxidant activity; Electrochemistry; Flow injection; DPPH•; Method comparison;
D O I：
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中图分类号：
学科分类号：
摘要：
An analytical method for simultaneous determination of total polyphenol content (TPC) and antioxidant activity (AA) of wines (white and red wines) and oenological tannins, using a flow injection system with sequential diode array and electrochemical amperometry detectors (DAD-ECD), was proposed. The signal at 280 nm provided aggregate data for TPC. The anodic peak related to wine phenolic oxidation was scanned using pulsed integrated amperometry over the potential of 800 mV vs. Ag/AgCl, to obtain AA. Serial dilutions avoided the poisoning at the glassy carbon (GC) electrode and the linear response obtained with both detectors was compared with spectrophotometric assays commonly used in oenology laboratory. Intraday and interday analytical repetitions showed a good repeatability and reproducibility (relative standard deviation RSD < 6% for both detectors), and the satisfactory relationship between the proposed coupled flow injection/DAD-ECD and the classic UV methods (R2TPC = 0.9967; R2DPPH = 0.9621) confirmed the efficacy of flow injection analysis with a coupled detection system, for the reliable quality control of wine and wine-related products.
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页码：347 / 354
页数：7
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