Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary

被引:0
作者
Akihiro Ohyama
Junko Toyomura
Toshiaki Tachibana
Seiji Isonishi
Haruka Takahashi
Hiroshi Ishikawa
机构
[1] The Nippon Dental University,Department of NDU Life Sciences, School of Life Dentistry
[2] The Jikei University School of Medicine,Division of Fine Morphology, Core Research Facilities
[3] Jikei Daisan Hospital,Department of Obstetrics and Gynecology
[4] The Nippon Dental University,Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata
来源
Human Cell | 2016年 / 29卷
关键词
Ovarian clear cell carcinoma; NOCC cell line; Transplantation; VEGF; HIF-1α;
D O I
暂无
中图分类号
学科分类号
摘要
A cell line, designated NOCC, was established from the ascites of a patient with clear cell adenocarcinoma of the ovary. The cell line has been grown without interruption and continuously propagated by serial passaging (more than 76 times) over 7 years. The cells are spherical to polygonal-shaped, display neoplastic, and pleomorphic features, and grow in a jigsaw puzzle-like pattern while forming monolayers without contact inhibition. The cells proliferate rapidly, but are easily floated as a cell sheet. The population doubling time is about 29 h. The number of chromosomes ranges from 60 to 83. The modal number of chromosomes is 70–74 at the 30th passage. NOCC cells secreted 750.5 ng/ml of VEGF over 3 days of culture. Hypoxia inducible factor-1α (HIF-1α) is a primary regulator of VEGF under hypoxic conditions. NOCC cells were not sensitive to the anticancer drugs BEV, DOX, GEM, ETP, CDDP, or TXT. The graft of NOCC cells to a scid mouse displayed similar histological aspects to the original tumor. Both the NOCC cells and the graft of the NOCC cells gave a positive PAS reaction.
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页码:188 / 196
页数:8
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