Hapten Synthesis and Monoclonal Antibody-Based Immunoassay Development for the Analysis of Thidiazuron

被引：0
作者：
Yongliang Cui
Zhen Cao
Suqin Guo
Wei Zhang
Guiyu Tan
Zhaohu Li
Baomin Wang
机构：
[1] China Agricultural University,College of Agriculture and Biotechnology
[2] China Agricultural University,College of Science
来源：
Journal of Plant Growth Regulation | 2016年 / 35卷
关键词：
Thidiazuron; Monoclonal antibody; Herbicide residue; Cotton defoliant; Immunoassay;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Thidiazuron (TDZ) is a synthetic plant growth regulator, which is widely used in agriculture and tissue culture. A sensitive and specific monoclonal antibody (mAb3D6F5B2) was obtained. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with mAb3D6F5B2. The established icELISA possessed a 50 % inhibition concentration (IC50) of 0.61 ng/mL and a quantitative range of 0.15–2.49 ng/mL. The recoveries of TDZ fortified in water and soil samples were 76–126 %. The residual level of TDZ in soil samples detected by icELISA was confirmed with high performance liquid chromatography-mass spectrometry (LC/MS). The correlation coefficient (R2) between the two assays was 0.9898, demonstrating this icELISA is suitable to detect TDZ residues in environmental and agricultural samples.
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页码：357 / 365
页数：8
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