Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering

被引：92

作者：

Antoun A. ^{[1
]}

Pavlov M.Y. ^{[1
]}

Tenson T. ^{[2
]}

Ehrenberg M. ^{[1
]}

机构：

[1] Department of Cell/Molec. Biology, BMC, Uppsala University, S-75 124 Uppsala

[2] Institute of Technology, Tartu University, Tartu 51010

来源：

Biological Procedures Online | 2004年 / 6卷 / 1期

关键词：

Prokaryotic initiation factor-2; Radiation; Ribosomes; Scattering;

D O I：

10.1251/bpo71

中图分类号：

学科分类号：

摘要：

Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions on subunit association were studied along with the role of GTP in this process. The part of light scattering theory that is essential for kinetic measurements is highlighted in the main text and a more general treatment of Rayleigh scattering from macromolecules is given in an appendix. © 2004. Biological Procedures Online.

引用

页码：35 / 54

页数：19

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