Optimization of Medium Components for Production of Cellulases by Melanocarpus sp. MTCC 3922 under Solid-state Fermentation

被引：0
作者：
K. Jatinder
B. S. Chadha
H. S. Saini
机构：
[1] Guru Nanak Dev University,Department of Microbiology
来源：
World Journal of Microbiology and Biotechnology | 2006年 / 22卷
关键词：
A vicelase; Avicel-adsorbable endoglucanase; Endoglucanase; FPase; -glucosidase; sp. MTCC 3922; Solid-state fermentation; Xylanase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The medium components for the production of extracellular cellulases by Melanocarpus sp. MTCC 3922 were optimized using solid-state fermentation. Melanocarpus sp. cultured in optimized medium containing 1.5% urea, and 0.12% KH2PO4 along with a trace element solution and surfactant (Tween 20), produced endoglucanase (142.4 U/g of substrate), Avicel-adsorbable endoglucanase (27.0 U/g of substrate), Avicelase (0.65 U/ g of substrate), FPase (39.9 U/g of substrate) and β-glucosidase (109.0 U/g of substrate) activities. The presence of sulphate ions in traces stimulated endoglucanase yields. The IEF fractionation of the crude proteins from Melanocarpus sp. showed the expression of 3, 1 and 11 isoforms of endoglucanase, β-glucosidase and xylanase, respectively.
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页码：15 / 22
页数：7
相关论文
共 56 条
[1]  
Arifoğlu N.(2000)Avicel adsorbable endoglucanase production by thermophilic fungus Enzyme and Microbial Technology 27 560-569
[2]  
Ögel Z.B.(2004) type culture Enzyme and Microbial Technology 35 460-466
[3]  
Badhan A.K.(2000)Functionally diverse multiple xylanases of thermophilic fungus Biotechnology Advances 18 355-383
[4]  
Chadha B.S.(1987) sp. IMI 387099 Enzyme and Microbial Technology 9 169-152
[5]  
Sonia K.G.(1998)Cellulase and related enzymes in biotechnology Carbohydrate Polymers 37 273-277
[6]  
Saini H.S.(2000b)Isolation and properties of mutants of the fungus Enzyme and Microbial Technology 27 664-671
[7]  
Bhat M.K.(1986) with enhanced cellulase and Applied and Environmental Microbiology 51 1041-1046
[8]  
Bhat M.K.(1995)-glucosidase production Applied Microbiology and Biotechnology 43 604-609
[9]  
Brown A.J.(2000)Mechanism of cellulase action in textile processes Microbiology and Molecular Biology Reviews 64 461-488
[10]  
Falconer D.J.(1985)Surface hydrophobic amino acid residue in cellulase molecules as a structural factor responsible for their high denim washing performance Journal of General Microbiology 131 3017-3027
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