Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics

被引:0
作者
Ede A Rancz
Kevin M Franks
Martin K Schwarz
Bruno Pichler
Andreas T Schaefer
Troy W Margrie
机构
[1] Physiology and Pharmacology,Department of Neuroscience
[2] University College London,Division of Neurophysiology
[3] The National Institute for Medical Research,Department of Neuroscience
[4] Columbia University,Department of Molecular Neurobiology
[5] Max Planck Institute for Medical Research,undefined
[6] SNWG Behavioural Neurophysiology,undefined
[7] Max Planck Institute for Medical Research,undefined
来源
Nature Neuroscience | 2011年 / 14卷
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学科分类号
摘要
The authors describe a technique for delivering DNA vectors through a patch-pipette following characterization of the neuron by whole-cell recording. They demonstrate the utility of the approach by mapping the synaptic and anatomical receptive fields of several visual cortical neurons.
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页码:527 / 532
页数:5
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