White matter preservation after spinal cord injury in ICAM-1/P-selection-deficient mice

被引：0

作者：

Farooque M. ^{[1
]}

Isaksson J. ^{[1
]}

Olsson Y. ^{[1
]}

机构：

[1] Research Group of Neuropathology, Department of Genetics and Pathology, Uppsala University Hospital

来源：

Acta Neuropathologica | 2001年 / 102卷 / 2期

关键词：

ICAM-1; Inflammation; Mouse; P-selectin; Spinal cord injury;

D O I：

10.1007/s004010000307

中图分类号：

学科分类号：

摘要：

We have previously demonstrated that mice deficient in ICAM-1 and P-selectin (ICAM-1/PS-/-) have improved functional recovery after spinal cord injury (SCI), compared to injured controls. In this study the spinal cords from wild-type and ICAM-1/PS-/- mice were evaluated histopathologically 14 days after severe compression-type SCI. Following injury there was an atrophy of the spinal cord. Significant sparing of total cross-sectional area was noted in ICAM-1/PS-/- mice compared to injured controls at the site of compression and in the distal peri-injury zone. Likewise, significant preservation of white matter area, as measured by Luxol staining, was found in mutant mice at the site of injury and in the proximal peri-injury zone. Gray matter damage was investigated by microtubule-associated protein 2 immunohistochemistry. Following severe SCI, a trend of gray matter sparing was noticed in ICAM-1/PS-/- animals. Quantitation of iba1 immunohistochemistry revealed that microglial reaction was significantly suppressed in the mutant animals. Astroglial reaction, visualized by GFAP immunostaining, did not differ between groups. Our results indicate that ICAM-1 and P-selectin are involved in autodestructive events provoked by the initial injury but the precise underlying mechanisms remain obscure.

引用

页码：132 / 140

页数：8

共 41 条

[1]

Blight A., Macrophages and inflammatory damage in spinal cord injury, J Neurotrauma, 9, pp. 83-91, (1992)

[2]

Bullard D., Qin L., Lorenzo I., Quinlin W., Doyle N., Bosse R., Vestweber D., Doerschuck C., Beaudet A., P-selectin/ICAM-1 double mutant mice: Acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli, J Clin Invest, 95, pp. 1782-1785, (1995)

[3]

Carlos T., Harlan J., Leukocyte-endothelial adhesion molecules, Blood, 84, pp. 2068-2101, (1994)

[4]

Clark R., Kochanek P., Schwarz M., Schiding J., Turner D., Chen M., Carlos T., Watkins S., Inducible nitric oxide synthase expression in cerebrovascular smooth muscle and neutrophils after traumatic brain injury in immature rats, Pediatr Res, 39, pp. 784-790, (1996)

[5]

Connolly E., Winfree C., Springer T., Naka Y., Liao H., Yan S., Stern D., Solomon R., Gutierrez-Ramos J.-C., Pinsky D., Cerebral protection in homozygous null ICAM-1 mice after middle cerebral artery occlusion, J Clin Invest, 97, pp. 209-216, (1996)

[6]

Connolly E., Winfree C., Prestigiacomo C., Kim S., Choudhri T., Hoh B., Naka Y., Solomon R., Pinsky D., Exacerbation of cerebral injury in mice that express the P-selectin gene, Circ Res, 81, pp. 304-310, (1997)

[7]

Farooque M., Spinal cord compression in the mouse: Presentation of a method including assessment of motor dysfunction, Acta Neuropathol, 100, pp. 13-22, (2000)

[8]

Farooque M., Zhang Y., Holtz A., Olsson Y., Exudation of fibronectin and albumin after spinal cord injury in rats, Acta Neuropathol, 84, pp. 613-620, (1992)

[9]

Farooque M., Badonic T., Olsson Y., Holtz A., Astrocytic reaction after graded spinal cord compression in rats: Immunohistochemical studies on glial fibrillary acidic protein and vimentin, J Neurotrauma, 12, pp. 41-51, (1995)

[10]

Farooque M., Isaksson J., Jackson D., Olsson Y., Clomethiazole (Zendra, CMZ) improves hind limb motor function and reduces neuronal damage after severe spinal cord injury in rat, Acta Neuropathol, 98, pp. 22-30, (1999)

← 1 2 3 4 5 →