Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

被引：0
作者：
Bin Jiang
Zhibiao Feng
Chunhong Liu
Yingcao Xu
Dongmei Li
Guo Ji
机构：
[1] Northeast Agricultural University,Department of Applied Chemistry
来源：
Journal of Food Science and Technology | 2015年 / 52卷
关键词：
Ionic liquid; Aqueous two-phase system; Wheat-esterase; Purification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To explore a new and simple rapid extraction and purification technique for wheat-esterase, an ionic liquids (ILs)-based aqueous two-phase system (ATPS) was developed for the purification of wheat-esterase from wheat extracts. Effects of various process parameters such as the concentrations of [Bmim]BF4, the types and concentrations of phase-forming salt, the system pH and the temperature on partitioning of wheat-esterase were evaluated. The obtained data indicated that wheat-esterase was preferentially partitioned into the ILs-rich phase and the ATPS composed of 20 % [Bmim]BF4 (w/w) and 25 % (w/w) NaH2PO4(pH = 4.8) showed good selectivity on wheat-esterase. Under the optimum conditions, wheat-esterase was purified with an acceptable yield (88.93 %), but produced wheat-esterase was 4.23 times as pure. It was obvious that temperature shows little influence on the purification between 10 and 50 °C. Sephadex G-150FF revealed that the band intensity of contaminating proteins in ATPS fraction almost disappeared. Therefore, ILs-based ATPS was an effective method for partitioning and recovery of wheat-esterase from wheat crude extracts.
引用
收藏
页码：2878 / 2885
页数：7
相关论文
共 79 条
[1]  
Aguilar O(2010)Aqueous two-phase systems strategies for the recovery and characterization of biological products from plants J Sci Food Agric 90 1385-1392
[2]  
Rito-Palomares M(1976)Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding Anal Biochem 72 248-254
[3]  
Bradford MM(2004)Purification and cloning of an esterase from the weed black-grass (Alopecurus myosuroides), which bioactivates aryloxyphenoxypropionate herbicides Plant J 39 894-904
[4]  
Cummins I(2003)Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae) Genet Mol Biol 26 195-198
[5]  
Edwards R(2010)Lysozyme extraction from hen egg white in an aqueous two-phase system composed of ethylene oxide-propylene oxide thermoseparating copolymer and potassium phosphate Process Biochem 45 369-374
[6]  
De Carvalho VM(2009)Driving forces of protein partitioning in an ionic liquid-based aqueous two-phase system Biochem Eng J 46 176-185
[7]  
Marques RM(2006)Partitioning of beta-glucosidase from Trichoderma reesei in poly (ethylene glycol) and potassium phosphate aqueous two-phase systems: influence of pH and temperature Biochem Eng J 30 104-108
[8]  
Lapenta AS(2003)Controlling the aqueous miscibility of ionic liquids: aqueous biphasic systems of water-miscible ionic liquids and water-structuring salts for recycle, metathesis, and separations J Am Chem Soc 125 6632-6633
[9]  
Machado M(2011)Room-temperature ionic liquids: solvents for synthesis and catalysis. 2 Chem Rev 111 3508-3576
[10]  
Dembczynski R(2009)Optical detection of dimethyl methyl-phosphonate with monosulfonate tetraphenyl porphyrin-plant-esterase complex Sens Lett 7 72-78
12345678