Immuno-Localization of CD44 and Osteopontin in Developing Human Kidney

被引：9

作者：

Crisi, Giovanna M. ^{[1
]}

Marconi, Sharon A. ^{[1
]}

Rockwell, Gary F. ^{[3
]}

Braden, Gregory L. ^{[2
]}

Campfield, Thomas J. ^{[3
]}

机构：

[1] Baystate Med Ctr, Dept Pathol, Springfield, MA 01199 USA

[2] Baystate Med Ctr, Dept Med, Springfield, MA 01199 USA

[3] Baystate Med Ctr, Dept Pediat, Springfield, MA 01199 USA

来源：

PEDIATRIC RESEARCH | 2009年 / 65卷 / 01期

关键词：

TUBULAR EPITHELIAL-CELLS; BRANCHING MORPHOGENESIS; CRYSTAL-BINDING; MOLECULAR REGULATION; PREMATURE-INFANTS; HYALURONIC-ACID; EXPRESSION; CALCIUM; INJURY; FETAL;

D O I：

10.1203/PDR.0b013e31818912b7

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

CD44 is observed ill ureteric bud structures and is implicated in branching morphogenesis during early mouse renal development. Healthy adult kidney demonstrates minimal CD44, but CD44 is up-regulated in renal diseases. CD44 may mediate binding of calcium oxalate crystals to tubular epithelia via the ligands osteopontin (OPN) and hyaluronan. Because 15% of premature infants develop nephrocalcinosis, developmental tubular CD44 expression might promote nephrocalcinosis. We Studied CD44 and OPN immuno-localization in developing human kidney by immunohistochemical analysis. Human renal tissue between 18 and 40 wk of-localization in ureteric buds, with gestation showed CD44 immuno-staining, decreasing with increasing gestational age; CD44 was rarely observed in developing renal tubules. OPN Was diffusely observed in proximal tubules, rarely observed in distal tubules, ureteric buds or mentanephric structures. These data Support the role of CD44 in early human nephron formation and branching morphogenesis. Rare CD44 staining in developing tubular epithelium suggests no role for CD44 in promoting Calcium oxalate adherence to tubular epithelia in premature infants. Immuno-localization of OPN in tubules supports its role in tubular differentiation, but OPN does not seem to be necessary during early nephron formation. (Pediatr Res 65: 79-84, 2009)

引用

页码：79 / 84

页数：6

共 44 条

[1] Osteopontin expression in acute renal allograft rejection [J].

Alchi, B ;

Nishi, S ;

Kondo, D ;

Kaneko, Y ;

Matsuki, A ;

Imai, N ;

Ueno, M ;

Iguchi, S ;

Sakatsume, M ;

Narita, I ;

Yamamoto, T ;

Gejyo, F .

KIDNEY INTERNATIONAL, 2005, 67 (03) :886-896

[2] CD44 IS THE PRINCIPAL CELL-SURFACE RECEPTOR FOR HYALURONATE [J].

ARUFFO, A ;

STAMENKOVIC, I ;

MELNICK, M ;

UNDERHILL, CB ;

SEED, B .

CELL, 1990, 61 (07) :1303-1313

[3] Calcium oxalate crystal adherence to hyaluronan-, osteopontin-, and CD44-expressing injured/regenerating tubular epithelial cells in rat kidneys [J].

Asselman, M ;

Verhulst, A ;

De Broe, ME ;

Verkoelen, CF .

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2003, 14 (12) :3155-3166

[4] Enhanced tubular epithelial CD44 expression in MRL-lpr lupus nephritis [J].

Benz, PS ;

Fan, XH ;

Wuthrich, RP .

KIDNEY INTERNATIONAL, 1996, 50 (01) :156-163

[5]

CAMPFIELD T, 1994, PEDIATRICS, V94, P674

[6]

CHEN Y, 1992, J BIOL CHEM, V267, P24871

[7] Molecular regulation of nephron endowment [J].

Clark, AT ;

Bertram, JF .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1999, 276 (04) :F485-F497

[8] Identification of osteopontin as a novel ligand for the integrin α8β1 and potential roles for this integrin-ligand interaction in kidney morphogenesis [J].

Denda, S ;

Reichardt, LF ;

Müller, U .

MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (06) :1425-1435

[9] RENAL CALCIFICATION IN PRETERM INFANTS - PATHO-PHYSIOLOGY AND LONG-TERM SEQUELAE [J].

EZZEDEEN, F ;

ADELMAN, RD ;

AHLFORS, CE .

JOURNAL OF PEDIATRICS, 1988, 113 (03) :532-539

[10] Upregulation of osteopontin expression in renal cortex of streptozotocin-induced diabetic rats is mediated by bradykinin [J].

Fischer, JW ;

Tschöpe, C ;

Reinecke, A ;

Giachelli, CM ;

Unger, T .

DIABETES, 1998, 47 (09) :1512-1518

← 1 2 3 4 5 →