Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity

被引:0
作者
Bai-Wen Fu
Lian Xu
Mei-Xia Zheng
Yan Shi
Yu-Jing Zhu
机构
[1] Fujian Academy of Agricultural Sciences,Institute of Crop Sciences
[2] Xiamen University,School of Life Sciences
来源
AMB Express | / 14卷
关键词
Cry2Ab; Protein engineering; Oligomerization; Pore-forming activity; Insecticidal activity;
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学科分类号
摘要
Bacillus thuringiensis Cry2Ab toxin was a widely used bioinsecticide to control lepidopteran pests all over the world. In the present study, engineering of Bacillus thuringiensis Cry2Ab toxin was performed for improved insecticidal activity using site-specific saturation mutation. Variants L183I were screened with lower LC50 (0.129 µg/cm2) against P. xylostella when compared to wild-type Cry2Ab (0.267 µg/cm2). To investigate the molecular mechanism behind the enhanced activity of variant L183I, the activation, oligomerization and pore-formation activities of L183I were evaluated, using wild-type Cry2Ab as a control. The results demonstrated that the proteolytic activation of L183I was the same as that of wild-type Cry2Ab. However, variant L183I displayed higher oligomerization and pore-formation activities, which was consistence with its increased insecticidal activity. The current study demonstrated that the insecticidal activity of Cry2Ab toxin could be assessed using oligomerization and pore-formation activities, and the screened variant L183I with improved activity might contribute to Cry2Ab toxin’s future application.
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