Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity
被引:0
作者:
Bai-Wen Fu
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机构:Fujian Academy of Agricultural Sciences,Institute of Crop Sciences
Bai-Wen Fu
Lian Xu
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h-index: 0
机构:Fujian Academy of Agricultural Sciences,Institute of Crop Sciences
Lian Xu
Mei-Xia Zheng
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h-index: 0
机构:Fujian Academy of Agricultural Sciences,Institute of Crop Sciences
Mei-Xia Zheng
Yan Shi
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h-index: 0
机构:Fujian Academy of Agricultural Sciences,Institute of Crop Sciences
Yan Shi
Yu-Jing Zhu
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h-index: 0
机构:Fujian Academy of Agricultural Sciences,Institute of Crop Sciences
Yu-Jing Zhu
机构:
[1] Fujian Academy of Agricultural Sciences,Institute of Crop Sciences
[2] Xiamen University,School of Life Sciences
来源:
AMB Express
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14卷
关键词:
Cry2Ab;
Protein engineering;
Oligomerization;
Pore-forming activity;
Insecticidal activity;
D O I:
暂无
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学科分类号:
摘要:
Bacillus thuringiensis Cry2Ab toxin was a widely used bioinsecticide to control lepidopteran pests all over the world. In the present study, engineering of Bacillus thuringiensis Cry2Ab toxin was performed for improved insecticidal activity using site-specific saturation mutation. Variants L183I were screened with lower LC50 (0.129 µg/cm2) against P. xylostella when compared to wild-type Cry2Ab (0.267 µg/cm2). To investigate the molecular mechanism behind the enhanced activity of variant L183I, the activation, oligomerization and pore-formation activities of L183I were evaluated, using wild-type Cry2Ab as a control. The results demonstrated that the proteolytic activation of L183I was the same as that of wild-type Cry2Ab. However, variant L183I displayed higher oligomerization and pore-formation activities, which was consistence with its increased insecticidal activity. The current study demonstrated that the insecticidal activity of Cry2Ab toxin could be assessed using oligomerization and pore-formation activities, and the screened variant L183I with improved activity might contribute to Cry2Ab toxin’s future application.
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Obata, Fumiaki
Kitami, Madoka
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Kitami, Madoka
Inoue, Yukino
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Inoue, Yukino
Atsumi, Shogo
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Atsumi, Shogo
Yoshizawa, Yasutaka
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Yoshizawa, Yasutaka
Sato, Ryoichi
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
机构:
UCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
Univ St Joseph Beyrouth, Fac Sci, UR EGP, Lab Biodivers & Funct Genom, Beirut, LebanonUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
Fayad, Nancy
Kambris, Zakaria
论文数: 0引用数: 0
h-index: 0
机构:
Amer Univ Beirut, Fac Arts & Sci, Biol Dept, Beirut, LebanonUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
Kambris, Zakaria
El Chamy, Laure
论文数: 0引用数: 0
h-index: 0
机构:
Univ St Joseph Beyrouth, Fac Sci, UR EGP, Lab Biodivers & Funct Genom, Beirut, LebanonUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
El Chamy, Laure
Mahillon, Jacques
论文数: 0引用数: 0
h-index: 0
机构:
UCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, BelgiumUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
Mahillon, Jacques
Awad, Mireille Kallassy
论文数: 0引用数: 0
h-index: 0
机构:
Univ St Joseph Beyrouth, Fac Sci, UR EGP, Lab Biodivers & Funct Genom, Beirut, LebanonUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Yu, Zhaoqing
Fu, Yang
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Shanghai Acad Agr Sci, Inst Edible Fungi, Natl Engn Res Ctr Edible Fungi, Shanghai, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Fu, Yang
Zhang, Wei
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Zhang, Wei
Zhu, Li
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Zhu, Li
Yin, Wen
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Yin, Wen
Chou, Shan-Ho
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Chou, Shan-Ho
He, Jin
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
Buentello-Wong, S.
Galan-Wong, L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
Galan-Wong, L.
Arevalo-Nino, K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
Arevalo-Nino, K.
Almaguer-Cantu, V.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
Almaguer-Cantu, V.
Rojas-Verde, G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Obata, Fumiaki
Kitami, Madoka
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Kitami, Madoka
Inoue, Yukino
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Inoue, Yukino
Atsumi, Shogo
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Atsumi, Shogo
Yoshizawa, Yasutaka
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
Yoshizawa, Yasutaka
Sato, Ryoichi
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, JapanTokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo 1848588, Japan
机构:
UCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
Univ St Joseph Beyrouth, Fac Sci, UR EGP, Lab Biodivers & Funct Genom, Beirut, LebanonUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
Fayad, Nancy
Kambris, Zakaria
论文数: 0引用数: 0
h-index: 0
机构:
Amer Univ Beirut, Fac Arts & Sci, Biol Dept, Beirut, LebanonUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
Kambris, Zakaria
El Chamy, Laure
论文数: 0引用数: 0
h-index: 0
机构:
Univ St Joseph Beyrouth, Fac Sci, UR EGP, Lab Biodivers & Funct Genom, Beirut, LebanonUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
El Chamy, Laure
Mahillon, Jacques
论文数: 0引用数: 0
h-index: 0
机构:
UCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, BelgiumUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
Mahillon, Jacques
Awad, Mireille Kallassy
论文数: 0引用数: 0
h-index: 0
机构:
Univ St Joseph Beyrouth, Fac Sci, UR EGP, Lab Biodivers & Funct Genom, Beirut, LebanonUCLouvain, Earth & Life Inst, Lab Food & Environm Microbiol, Louvain La Neuve, Belgium
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Yu, Zhaoqing
Fu, Yang
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Shanghai Acad Agr Sci, Inst Edible Fungi, Natl Engn Res Ctr Edible Fungi, Shanghai, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Fu, Yang
Zhang, Wei
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Zhang, Wei
Zhu, Li
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Zhu, Li
Yin, Wen
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Yin, Wen
Chou, Shan-Ho
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
Chou, Shan-Ho
He, Jin
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R ChinaHuazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
Buentello-Wong, S.
Galan-Wong, L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
Galan-Wong, L.
Arevalo-Nino, K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
Arevalo-Nino, K.
Almaguer-Cantu, V.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico
Almaguer-Cantu, V.
Rojas-Verde, G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, MexicoUniv Autonoma Nuevo Leon, Inst Biotechnol, Fac Ciencias Biol, San Nicolas De Los Garza 66450, Nuevo Leon, Mexico