Pseudomonas linyingensis sp. nov.: A Novel Bacterium Isolated from Wheat Soil Subjected to Long-term Herbicides Application

A strain of genus Pseudomonas, LYBRD3-7T was isolated from long-term sulfonylurea herbicides applied wheat-field soil in Linying located in Henan province of China. This strain is a strictly aerobic and Gram-negative short rod-shaped bacterium with single flagellum. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this isolate as a member of Pseudomonas, and most closely to Pseudomonas tuomuerensis CGMCC 1.1365T (97.1 %) and P. alcaligenes IAM12411T (97.1 %). Morphological characters and chemotaxonomic data confirmed the affiliation of strain LYBRD3-7T to the genus Pseudomonas. The results of phylogenetic analysis, physiological and biochemical studies, and DNA–DNA hybridization allowed the differentiation of genotype and phenotype between strain LYBRD3-7T and the phylogenetic closest species with valid names. The name proposed for the new species is Pseudomonas linyingensis sp. nov. The type strain is LYBRD3-7T (=CGMCC 1.10701T  =LMG 25967T).