Lysozyme refolding at high concentration by dilution and size-exclusion chromatography

被引：13

作者：

Yong-gui Gao

Yi-xin Guan

Shan-jing Yao

Man-gi Cho

机构：

[1] Dept. of Chem. and Biochem. Eng., Zhejiang Univ.

[2] Coll. of Life Sci., Zhejiang Univ.

[3] Eng. Res. Cent., Dong-Seo Univ., Busan 617-716

来源：

Journal of Zhejiang University-SCIENCE A | 2003年 / 4卷 / 2期

关键词：

Dilution; Downstream process; Lysozyme refolding; Size exclusion chromatography; Urea;

D O I：

10.1631/jzus.2003.0136

中图分类号：

学科分类号：

摘要：

This study of renaturation by dilution and size exclusion chromatography (SEC) addition of urea to improve yield as well as the initial and final protein concentrations showed that although urea decreased the rate of lysozyme refolding, it could suppress protein aggregation to sustain the pathway of correct refolding at high protein concentration; and that there existed an optimum urea concentration in renaturation buffer. Under the above conditions, lysozyme was successfully refolded from initial concentration of up to 40 mg/mL by dilution and 100 mg/mL by SEC, with the yield of the former being more than 40% and that of the latter being 34.8%. Especially, under the condition of 30 min interval time, i.e. τ>2(tR2 - tR1), the efficiency was increased by 25% and the renaturation buffer could be recycled for SEC refolding in continuous operation of downstream process.

引用

页码：136 / 141

页数：5

共 14 条

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