Expression of the chitinase gene from Trichoderma aureoviride in Saccharomyces cerevisiae

被引：0

作者：

Song Jinzhu

Yang Qian

Liu Beidong

Chen Dianfu

机构：

[1] Harbin Institute of Technology,Department of Life Science and Engineering

来源：

Applied Microbiology and Biotechnology | 2005年 / 69卷

关键词：

Chitin; Chitinase; Trichoderma; Raffinose; Chitinase Activity;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Chitinase gene ech42 was obtained from Trichoderma aureoviride M and amplified by PCR. The isolated DNA of ech42 was then sequenced. The results showed that the open reading frame of ech42 was 1,447 bp long, encoding 421 amino acids. Three introns were found in the sequence. The cloning vector pMD18-T and an E. coli DH5α host were used to yield clones as E. coli DH5α/ech42. The ech42 gene was integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/ech42. Chitinase expressed by pYES2/ech42 was induced by galactose (maximal activity 0.50 units ml−1) and was produced in fermentation liquid cultured for 36 h.

引用

页码：39 / 43

页数：4

共 50 条

[41] Stable Integration and Expression of Chitinase Gene in Transgenic Tomato
Mythili, J. B.
Rajeev, P. R.
Ganeshan, G.
Chowdappa, P.
Yadav, S.
Gowda, P. H. Ramanjini
XXVIII INTERNATIONAL HORTICULTURAL CONGRESS ON SCIENCE AND HORTICULTURE FOR PEOPLE (IHC2010): INTERNATIONAL SYMPOSIUM ON GENOMICS AND GENETIC TRANSFORMATION OF HORTICULTURAL CROPS, 2012, 929 : 373 - 379
[42] Characterisation and developmental expression of a chitinase gene in Heterodera glycines
Gao, BL
Allen, R
Maier, T
McDermott, JP
Davis, EL
Baum, TJ
Hussey, RS
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2002, 32 (10) : 1293 - 1300
[43] Cloning and expression of a chitinase gene from Thermoactinomyces vulgaris KFB-C100
Yoon, HG
Kim, HY
Lim, YH
Cho, HY
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 8 (06) : 560 - 567
[44] Correct targeting of a vacuolar tobacco chitinase in Saccharomyces cerevisiae -: post-translational modifications are dependent on the host strain
Kunze, I
Nilsson, C
Adler, K
Manteuffel, R
Horstmann, C
Bröker, M
Kunze, G
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1998, 1395 (03): : 329 - 344
[45] Purification of a chitinase from Trichoderma sp. and its action on Sclerotium rolfsii and Rhizoctonia solani cell walls
Lima, LHC
Ulhoa, CJ
Fernandes, AP
Felix, CR
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1997, 43 (01) : 31 - 37
[46] Candida albicans CHT3 encodes the functional homolog of the Cts1 chitinase of Saccharomyces cerevisiae
Dünkler, A
Walther, A
Specht, CA
Wendland, J
FUNGAL GENETICS AND BIOLOGY, 2005, 42 (11) : 935 - 947
[47] Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and Virus
Parveen, Samia
Khan, Anwar
Jahan, Nusrat
Aaliya, Khadija
Muzaffar, Adnan
Tabassum, Bushra
Inayatullah, Syed
Moeezullah, Syed
Tariq, Muhammad
Rehmat, Zainia
Ali, Niaz
Hussain, Abrar
GENES, 2023, 14 (05)
[48] Gene expression in endoprosthesis loosening: Chitinase activity for early diagnosis?
Morawietz, L.
Weimann, A.
Schroeder, J. H.
Kuban, R. J.
Ungethuem, U.
Kaps, C.
Slevogt, H.
Gehrke, T.
Krukemeyer, M. G.
Krenn, V.
JOURNAL OF ORTHOPAEDIC RESEARCH, 2008, 26 (03) : 394 - 403
[49] Expression and characterization of a chitinase from Serratia marcescens
Li, Jincheng
Zheng, Jiamin
Liang, Yanhui
Yan, Renxiang
Xu, Xinqi
Lin, Juan
PROTEIN EXPRESSION AND PURIFICATION, 2020, 171
[50] Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea
Meng, Huimin
Wang, Zhangxun
Meng, Xiangyun
Xie, Ling
Huang, Bo
GENETICS AND MOLECULAR BIOLOGY, 2015, 38 (03) : 381 - 389

← 1 2 3 4 5 →