Induction of human cytotoxic T lymphocytes by artificial antigen-presenting cells

被引：0
作者：
Jean-Baptiste Latouche
Michel Sadelain
机构：
[1] Memorial Sloan-Kettering Cancer Center,Department of Human Genetics Immunology Program
来源：
Nature Biotechnology | 2000年 / 18卷
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摘要：
The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts were retrovirally transduced with a single HLA–peptide complex along with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit strong stimulation and expansion of HLA-restricted CTLs. Owing to the high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily engineered for any HLA molecule and any specific peptide.
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页码：405 / 409
页数：4
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