Elimination of cells with abnormal nuclei in human epidermoid carcinoma cell line A431 by α-lipoic acid

被引：0
作者：
Kisurina-Evgenieva O.P. [1 ]
Onishchenko G.E. [1 ]
机构：
[1] Department of Cell Biology and Histology, Moscow State University, Moscow
来源：
Cell and Tissue Biology | 2010年 / 4卷 / 2期
基金：
俄罗斯基础研究基金会;
关键词：
α-lipoic acid; Apoptosis; Epidermoid carcinoma cell line;
D O I：
10.1134/S1990519X10020070
中图分类号：
学科分类号：
摘要：
Skin is usually exposed to adverse environmental conditions that may cause pathological cell proliferation and malignant transformation. Antioxidants are able to affect these processes and eliminate transformed cells. The purpose of this work was to investigate the effect of α-lipoic acid (ALA) on human epidermoid carcinoma cell line A431. It was found that 100, 200, 300, 500 μM ALA added for 24, 48, 72 h inhibited cell proliferation and stimulated apoptosis. Most dying cells have abnormal nuclei (micronuclei, giant nuclei, nuclei with buds). Electron microscopy showed that cells with normal nuclear phenotypes after treatment with 200 μM ALA for 48 h had ultrastructural organizations typical for control cells. Thus, α-ALA not only triggers the apoptosis of carcinoma cells, but it may also activate the mechanism for eliminating cells with abnormal numbers of chromosomes. © 2010 Pleiades Publishing, Ltd.
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页码：177 / 184
页数：7
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共 47 条
  • [1] Agarwal M.L., Taylor W.R., Chernov M.V., Chernova O.B., George R., Stark G.R., The p53 Network, J. Biol. Chem., 273, pp. 1-4, (1998)
  • [2] Bilska L., Wtodek L., Lipoic Acid-the Drug or the Future?, Pharm. Reports, 57, pp. 570-577, (2005)
  • [3] Brash D.E., Havre P.A., New Careers for Antioxidants, Proc. Natl. Acad. Sci. USA, 99, pp. 13969-13971, (2002)
  • [4] Byun C.H., Koh J.M., Kim D.K., Park S.I., Lee K.U., Kim G.S., Alpha-Lipoic Acid Inhibits TNF-AlphaInduced Apoptosis in Human Bone Marrow Stromal Cells, J. Bone Miner Res., 20, pp. 1125-1135, (2005)
  • [5] Cakatay U., Pro-Oxidant Actions of Alpha-Lipoic Acid and Dihydrolipoic Acid, Med. Hypotheses, 66, 1, pp. 110-117, (2006)
  • [6] Chesnokova N.P., Ponukalina E.V., Bizenkova M.N., General Characteristic of the Sources of Generation of Free Radicals and Antioxidant Systems. Advances in Modern Natural Science, Med. Nauki, 7, pp. 37-41, (2006)
  • [7] Dovinova I., Novotny L., Rauko P., Kvasnicka P., Combined Effect of Lipoic Acid and Doxorubicin in Murine Leukemia, Neoplasma, 46, pp. 237-241, (1999)
  • [8] Droge W., Free Radicals in the Physiological Control of Cell Function, Physiol. Rev., 82, pp. 47-95, (2001)
  • [9] Fenech M., The Cytokinesis-Block Micronucleus Technique and Its Application to Genotoxicity Studies in Human Populations, Env. Health Perspect. Suppl., 101, pp. 101-107, (1994)
  • [10] Grady W.M., Genomic Instability and Colon Cancer, Cancer Metastasis Rev., 23, pp. 11-27, (2004)
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