Nitric oxide participation in granulomatous response induced by Paracoccidioides brasiliensis infection in mice

被引：0
作者：
Angela Satie Nishikaku
Raphael Fagnani Sanchez Molina
Luciana Cristina Ribeiro
Renata Scavone
Bernardo Paulo Albe
Cláudia Silva Cunha
Eva Burger
机构：
[1] Universidade de São Paulo,Departamento de Imunologia, Instituto de Ciências Biomédicas
[2] Universidade Federal do Espírito Santo,Departamento de Ciências da Saúde, Biológicas e Agrárias, CEUNES
来源：
Medical Microbiology and Immunology | 2009年 / 198卷
关键词：
Experimental paracoccidioidomycosis; Inducible nitric oxide synthase; Granulomas; Osteopontin; Matrix metalloproteinases;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The role of nitric oxide (NO) in granulomas of Paracoccidioides brasiliensis-infected inducible NO synthase-deficient C57BL/6 mice (iNOS KO) and their wild-type counterparts and its association with osteopontin (OPN) and matrix metalloproteinases (MMPs) was studied. At 15 days after infection (DAI), iNOS KO mice showed compact and necrotic granulomas with OPN+ macrophages and multinucleated giant cells, whereas wild-type mice developed loose granulomas with many fungi and OPN+ cells distributed throughout the tissue. In addition, high OPN levels and fungal load were observed in iNOS KO mice. Both experimental groups had MMP-9 activity. At 120 DAI, iNOS KO had smaller granulomas with OPN+ cells, lower OPN levels, lower fungal load and decreased MMP-9 activity compared with wild-type mice. These findings suggest that NO has an important role in granuloma modulation, by controlling OPN and MMP production, as well as by inducing loose granulomas formation and fungal dissemination, resulting, at later phases, in progression of paracoccidioidomycosis.
引用
收藏
页码：123 / 135
页数：12
相关论文
共 188 条
  • [1] Batista AC(2005)Failure of nitric oxide production by macrophages and decrease in CD4 Rev Inst Med Trop Sao Paulo 47 267-273
  • [2] Soares CT(1998) T cells in oral paracoccidiodomycosis: possible mechanisms that permit local fungal multiplication J Immunol 161 3056-3063
  • [3] Lara VS(2001)Treatment of Nat Immunol 2 907-916
  • [4] Bocca AL(1996)-infected mice with a nitric oxide inhibitor prevents the failure of cell-mediated immune response Am J Trop Med Hyg 55 235-242
  • [5] Hayashi EE(1985)Nitric oxide and the immune response Br J Exp Pathol 66 585-599
  • [6] Pinheiro AG(2000)Histopathology of paracoccidioidomycotic infection in athymic and euthymic mice: a sequential study Rev Iberoam Micol 17 41-48
  • [7] Furlanetto AB(2005)Susceptibility and resistance of inbred mice to Biochem Biophys Res Commun 326 45-51
  • [8] Campanelli AP(1999)Current knowledge on pathogenesis and immunodiagnosis of paracoccidioidomycosis Immunology 98 313-323
  • [9] Cunha FQ(2005)Deficiency of inducible nitric oxide synthase exacerbates hepatic fibrosis in mice fed high-fat diet Clin Exp Immunol 142 12-20
  • [10] Figueiredo F(1995)NOS2-derived nitric oxide regulates the size, quantity and quality of granuloma formation in Acta Orthop Scand 266 61-65
12345678910