LncRNA MEG3 inhibits the inflammatory response of ankylosing spondylitis by targeting miR-146a

被引：0
作者：
Yehong Li
Shanshan Zhang
Cunxin Zhang
Meihong Wang
机构：
[1] Jining No.1 People’s Hospital (Jining First People’s Hospital Affiliated to Jining Medical University),Department of Spinal
[2] Jining No.1 People’s Hospital (Jining First People’s Hospital Affiliated to Jining Medical University),Surgery
[3] Jining No.1 People’s Hospital (Jining First People’s Hospital Affiliated to Jining Medical University),Department of Neurosurgery
来源：
Molecular and Cellular Biochemistry | 2020年 / 466卷
关键词：
Ankylosing spondylitis; MEG3; miR-146a; Inflammatory response; Cytokines;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Ankylosing spondylitis (AS) is a progressive systemic disease characterized by chronic inflammation response of the sacroiliac joint and spine. Long non-coding RNAs (lncRNAs) are widely involved in the regulation of various diseases. However, the role of lncRNA maternally expressed gene 3 (MEG3) in the inflammatory response of AS has not been studied. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of inflammatory cytokines interleukin-1β (IL-1β) and interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in tissues and cells. The expression levels of MEG3, microRNA-146a (miR-146a), and inflammatory cytokines were measured by quantitative real-time PCR (qRT-PCR). Correlation between MEG3 or miR-146a and inflammatory cytokines was analyzed by Pearson analysis. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were used to clarify the interaction between MEG3 and miR-146a. MEG3 was downregulated in AS patients, negatively correlated with the levels of IL-1β, IL-6, and TNF-α, and blocked the inflammatory response of AS. MiR-146a was upregulated in AS patients and could interact with MEG3. The expression of miR-146a was positively correlated with IL-1β, IL-6, and TNF-α levels. Overexpression of miR-146a reversed the inhibitory effect of abnormal MEG3 expression on inflammatory cytokines. LncRNA MEG3 plays an anti-inflammatory role in AS partially through targeting miR-146a, which provides a potential new means for the treatment of AS patients.
引用
收藏
页码：17 / 24
页数：7
相关论文
共 169 条
[21]  
Kwok ZH(2014)Treating spondyloarthritis, including ankylosing spondylitis and psoriatic arthritis, to target: recommendations of an international task force Ann Rheum Dis 4 5468-1192
[22]  
Tay Y(2011)Spondyloarthritis Lancet 77 63-12486
[23]  
Tay Y(2015)TNF-alpha inhibitors for ankylosing spondylitis Cochrane Database Syst Rev 373 2534-742
[24]  
Rinn J(2018)TNF blockers inhibit spinal radiographic progression in ankylosing spondylitis by reducing disease activity: results from the Swiss Clinical Quality Management cohort Ann Rheum Dis 43 1179-1249
[25]  
Pandolfi PP(2015)Secukinumab, an interleukin-17A inhibitor, in ankylosing spondylitis N Engl J Med 103 12481-undefined
[26]  
Xie Z(2019)Identification of the key genes and long noncoding RNAs in ankylosing spondylitis using RNA sequencing Int J Mol Med 9 7212-undefined
[27]  
Li J(2006)NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses Proc Natl Acad Sci U S A 10 e0122055-undefined
[28]  
Wang P(2019)Vibrio cholerae derived outer membrane vesicles modulate the inflammatory response of human intestinal epithelial cells by inducing microRNA-146a Sci Rep 41 735-undefined
[29]  
Li Y(2015)Association between ankylosing spondylitis and the miR-146a and miR-499 polymorphisms PLoS ONE ONE 97 1243-undefined
[30]  
Wu X(2016)Identification of Serum miR-146a and miR-155 as Novel Noninvasive Complementary Biomarkers for Ankylosing Spondylitis SPINE undefined undefined-undefined
12345678910