Adeno-associated virus serotype 2 mediated transduction and coexpression of the human apoAI and SR-BI gene in HepG2 cells

被引：0
作者：
Bingnan Li
Juan Zhang
Zhiyan Li
Mengqun Tan
机构：
[1] Xiang-Ya School of Medicine,Experimental Hematology Laboratory, Department of Physiology
[2] Central South University,undefined
[3] Shenzhen Institute of Xiangya Biomedicine,undefined
来源：
Molecular Biology Reports | 2012年 / 39卷
关键词：
Adeno-associated virus; Gene transfer; Apolipoprotein A-I; Scavenger receptor class B type I; Reverse cholesterol transport (RCT); Expression;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Cholesterol efflux is the first step in the reverse cholesterol transport (RCT) pathway, removing excess cholesterol from tissues, including the arterial wall, thus preventing the development of atherosclerosis. Adeno-associated virus (rAAV) has demonstrated significant promise as a DNA-delivery vector to treat serious human diseases. In this study, we constructed recombinant adeno-associated viruses coexpressing apoAI and SR-BI successfully, the double gene mRNA and protein were both strongly expressed in transduced HepG2 cells. A novel safe and efficient method of promoting the reverse cholesterol transport (RCT) may be established. These results may provide a new method for gene therapy of Arteriosclerosis.
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页码：25 / 32
页数：7
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