Fluorescence Quenching to Study Protein-ligand Binding: Common Errors

被引:0
|
作者
Marco van de Weert
机构
[1] University of Copenhagen,Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences
来源
Journal of Fluorescence | 2010年 / 20卷
关键词
Fluorescence quenching; FRET; Ligand binding; Inner-filter effect;
D O I
暂无
中图分类号
学科分类号
摘要
A number of recent articles, amongst others several published in the Journal of Fluorescence, use inappropriate fluorescence methodology to determine ligand binding characteristics to (mostly) proteins. In this Letter, several common pitfalls are discussed in relation to two recent publications in the Journal of Fluorescence (Wang et al. (2009) 19:801–808; Ding et al. (2009) 19:783–791). The Author hopes that this contribution helps to prevent a further spread of the incorrect methodology, and results in a reappraisal of those articles already published using similar methodology.
引用
收藏
页码:625 / 629
页数:4
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