Quantitative assessment of RNA-protein interactions with high-throughput sequencing–RNA affinity profiling

被引：0

作者：

Abdullah Ozer

Jacob M Tome

Robin C Friedman

Dan Gheba

Gary P Schroth

John T Lis

机构：

[1] Cornell University,Department of Molecular Biology and Genetics

[2] Molecular Microbial Pathogenesis Unit,undefined

[3] Institut Pasteur,undefined

[4] Illumina,undefined

来源：

Nature Protocols | 2015年 / 10卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This protocol is for high-throughput sequencing–RNA affinity profiling (HiTS-RAP). The technique uses an unmodified Illumina GAIIx sequencer to analyze millions of RNA-protein binding affinities in a single run.

引用

页码：1212 / 1233

页数：21

共 50 条

[21] Small noncoding RNA discovery and profiling with sRNAtools based on high-throughput sequencing
Liu, Qi
Ding, Changjun
Lang, Xiaoqiang
Guo, Ganggang
Chen, Jiafei
Su, Xiaohua
BRIEFINGS IN BIOINFORMATICS, 2021, 22 (01) : 463 - 473
[22] Profiling of RNA editing events in plant organellar transcriptomes with high-throughput sequencing
Liu, Kejia
Xie, Bin
Peng, Leilei
Wu, Qijia
Hu, Jun
PLANT JOURNAL, 2024, 118 (02): : 345 - 357
[23] High-throughput transcriptomic profiling of mRNAs and lncRNAs in the dermatomyositis muscle by RNA sequencing
Li, Ke
Ma, Yubao
Zhang, Shanchun
Ban, Rui
Shi, Qiang
RHEUMATOLOGY & AUTOIMMUNITY, 2023, 3 (03): : 166 - 178
[24] High throughput platform to explore RNA-protein interactomes
Sutandy, F. X. Reymond
Hsiao, Felix Shih-Hsiang
Chen, Chien-Sheng
CRITICAL REVIEWS IN BIOTECHNOLOGY, 2016, 36 (01) : 11 - 19
[25] Origins of the enhanced affinity of RNA-protein interactions triggered by RNA phosphorodithioate backbone modification
Yang, Xianbin
Abeydeera, N. Dinuka
Liu, Feng-Wu
Egli, Martin
CHEMICAL COMMUNICATIONS, 2017, 53 (76) : 10508 - 10511
[26] High-throughput profiling of peptide-RNA interactions using peptide microarrays
Yu, J. (jhoonyu@snu.ac.kr), 1600, American Chemical Society (134):
[27] High-Throughput Profiling of Peptide-RNA Interactions Using Peptide Microarrays
Pai, Jaeyoung
Yoon, Taejin
Kim, Nam Doo
Lee, Im-Soon
Yu, Jaehoon
Shin, Injae
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (46) : 19287 - 19296
[28] Contribution of RNA conformation to the stability of a high-affinity RNA-protein complex
Luchansky, SJ
Nolan, SJ
Baranger, AM
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (29) : 7130 - 7131
[29] Transient RNA-protein interactions in RNA folding
Doetsch, Martina
Schroeder, Renee
Fuertig, Boris
FEBS JOURNAL, 2011, 278 (10) : 1634 - 1642
[30] Profiling status epilepticus-induced changes in hippocampal RNA expression using high-throughput RNA sequencing
Hansen, Katelin F.
Sakamoto, Kensuke
Pelz, Carl
Impey, Soren
Obrietan, Karl
SCIENTIFIC REPORTS, 2014, 4

← 1 2 3 4 5 →