Quantitative assessment of RNA-protein interactions with high-throughput sequencing–RNA affinity profiling

被引：0
|
作者：
Abdullah Ozer
Jacob M Tome
Robin C Friedman
Dan Gheba
Gary P Schroth
John T Lis
机构：
[1] Cornell University,Department of Molecular Biology and Genetics
[2] Molecular Microbial Pathogenesis Unit,undefined
[3] Institut Pasteur,undefined
[4] Illumina,undefined
来源：
Nature Protocols | 2015年 / 10卷
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D O I：
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学科分类号：
摘要：
This protocol is for high-throughput sequencing–RNA affinity profiling (HiTS-RAP). The technique uses an unmodified Illumina GAIIx sequencer to analyze millions of RNA-protein binding affinities in a single run.
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页码：1212 / 1233
页数：21
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