Colorimetric thrombin assay using aptamer-functionalized gold nanoparticles acting as a peroxidase mimetic

被引：0
作者：
Laiping Zhang
Li Li
机构：
[1] Xinxiang University,College of Chemistry and Chemical Engineering
来源：
Microchimica Acta | 2016年 / 183卷
关键词：
Visual detection; Nanoparticles; Serum assay; Tetramethylbenzidine; Enzyme-like nanomaterial;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The article describes a method for the determination of thrombin that is based on the use of gold nanoparticles (AuNPs) functionalized with an aptamer labeled with a horseradish peroxidase mimick. The assay is based on (a) the high affinity between aptamer and thrombin, and (b) on the finding that AuNPs display HRP-like properties in catalyzing the oxidation of 3,3,5,5-tetramethylbenzidine to give a blue reaction product with an absorption maximum at 652 nm. The limit of detection for thrombin is as low as 0.02 pM, and the analytical range extends from 0.1 pM to 0.1 μM thrombin. The aptamer-based assay was successfully applied to the quantitation of thrombin in spiked human serum. This method is simple, inexpensive and highly sensitive.
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页码：485 / 490
页数：5
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共 164 条
[1]  
Stubbs MT(1993)A player of many parts: the spotlight falls on thrombin’s structure Thromb Res 69 1-58
[2]  
Bode W(2007)Aptamer-based detection of plasma proteins by an electrochemical assay coupled to magnetic beads Anal Chem 79 1466-1473
[3]  
Centi S(1995)Thrombin receptor activation protects neurons and astrocytes from cell death produced by environmental insults J Neurosci 15 5389-5401
[4]  
Tombelli S(2000)Simple and sensitive aptamer-based colorimetric sensing of protein using unmodified gold nanoparticle probes Proc Natl Acad Sci U S A 97 2264-3737
[5]  
Minunni M(2007)Optical sensors based on hybrid aptamer/conjugated polymer complexes Chem Commun 36 3735-1387
[6]  
Mascini M(2004)Detection of thrombin using electrogenerated chemiluminescence based on Ru(bpy) J Am Chem Soc 126 1384-248
[7]  
Vaughan PJ(2007)( Anal Chim Acta 598 242-13
[8]  
Pike CJ(2012))-doped silica nanoparticle aptasensor via target protein-induced strand displacement Talanta 98 7-12793
[9]  
Cotman CW(2009)Label-free electrochemical aptasensor for sensitive thrombin detection using layer-by-layer self-assembled multilayers with toluidine blue-graphene composites and gold nanoparticles Langmuir 25 12787-219
[10]  
Cunningham DD(2008)Conjugated polyelectrolyte-sensitized fluorescent detection of thrombin in blood serum using aptamer-immobilized silica nanoparticles as the platform Anal Biochem 373 213-2166
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