Determination of superoxide dismutase and SOD-mimetic activities by a chemical system: Co2/H2O2/lucigenin

被引：0
作者：
T. V. Zhidkova
E. V. Proskurnina
E. A. Parfenov
Yu. A. Vladimirov
机构：
[1] Lomonosov Moscow State University,Faculty of Fundamental Medicine
[2] N.N. Blokhin Russian Cancer Research Center,undefined
来源：
Analytical and Bioanalytical Chemistry | 2011年 / 401卷
关键词：
Superoxide dismutase/*analysis; Chemiluminescent measurements; Lucigenin; SOD mimetics; Animals;
D O I：
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学科分类号：
摘要：
The bright chemiluminescence has been observed in the system: Co2+/hydrogen peroxide/lucigenin. The chemiluminescence intensity was directly proportional to either cobalt, hydrogen peroxide, or lucigenin concentrations. A procedure of determination of superoxide dismutase (SOD) activity by the chemiluminescence method in the cobalt–hydrogen peroxide–lucigenin system at pH 8.5 is suggested. A linear dependence was established between a relative chemiluminescence intensity and SOD concentration in the range of SOD concentrations between 0 and 4.5 nM, c1/2 = 0.8 nM. The determination of SOD activity was performed in several tissue samples (rat plasma, erythrocyte hemolysate, and liver mitochondria). A technique of tissue sample preparation with the use of thermal inactivation of interfering proteins at 60 °C was used. The method was successfully applied for comparison of the efficiency of SOD mimetics.
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页码：381 / 386
页数：5
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