Plasmodium falciparum: a simplified technique for obtaining singly infected erythrocytes

被引:0
作者
Manoj K. Puthia
Kevin S. W. Tan
机构
[1] National University of Singapore,Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Faculty of Medicine
来源
Parasitology Research | 2005年 / 95卷
关键词
Malaria; Plasmodium Falciparum; Infected Erythrocyte; Thin Blood Smear; Rotation Culture;
D O I
暂无
中图分类号
学科分类号
摘要
We report the development of a simple technique involving 15 ml polypropylene tubes and a rotatory incubator for obtaining erythrocytes singly infected with Plasmodium falciparum. This technique will be useful for cloning of the parasite. Our finding that P. falciparum merozoite invasion is inhibited during rotation suggests that this method may also be useful for the study of parasite-erythrocyte interactions under dynamic circulatory conditions.
引用
收藏
页码:176 / 178
页数:2
相关论文
共 50 条
[41]   Plasmodium falciparum:: Influence of malarial and host erythrocyte skeletal protein interactions on phosphorylation in infected erythrocytes [J].
Magowan, C ;
Liang, J ;
Yeung, J ;
Takakuwa, Y ;
Coppel, RL ;
Mohandas, N .
EXPERIMENTAL PARASITOLOGY, 1998, 89 (01) :40-49
[42]   In vitro sensitivity of artemeter in Plasmodium falciparum-infected beta-thalassaemic trait erythrocytes [J].
Senok, AC ;
Li, K ;
Nelson, EAS ;
Chung, KW .
PARASITOLOGY, 1999, 118 :145-149
[43]   Surface antigens of Plasmodium falciparum-infected erythrocytes as immune targets and malaria vaccine candidates [J].
Chan, Jo-Anne ;
Fowkes, Freya J. I. ;
Beeson, James G. .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2014, 71 (19) :3633-3657
[44]   Acquisition of antibodies to variant antigens on the surface of Plasmodium falciparum-infected erythrocytes during pregnancy [J].
Fievet, Nadine ;
Le Hesran, Jean Yves ;
Cottrell, Gilles ;
Doucoure, Souleymane ;
Diouf, Ibrahima ;
Ndiaye, Jean Louis ;
Bertin, Gwladys ;
Gaye, Oumar ;
Sow, Sokhna ;
Deloron, Philippe .
INFECTION GENETICS AND EVOLUTION, 2006, 6 (06) :459-463
[45]   Disruption of erythrocyte rosettes and agglutination of erythrocytes infected with Plasmodium falciparum by the sera of Papua New Guineans [J].
Rogerson, SJ ;
Beck, HP ;
AlYaman, F ;
Currie, B ;
Alpers, MP ;
Brown, GV .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1996, 90 (01) :80-84
[46]   Palmitoylated Proteins in Plasmodium falciparum-Infected Erythrocytes: Investigation with Click Chemistry and Metabolic Labeling [J].
Kilian, Nicole ;
Zhang, Yongdeng ;
LaMonica, Lauren ;
Hooker, Giles ;
Toomre, Derek ;
Mamoun, Choukri Ben ;
Ernst, Andreas M. .
BIOESSAYS, 2020, 42 (06)
[47]   Stringent Selection of Knobby Plasmodium falciparum-Infected Erythrocytes during Cytoadhesion at Febrile Temperature [J].
Doerpinghaus, Michael ;
Fuerstenwerth, Finn ;
Roth, Lisa K. ;
Bouws, Philip ;
Rakotonirinalalao, Maximilian ;
Jordan, Vincent ;
Sauer, Michaela ;
Rehn, Torben ;
Pansegrau, Eva ;
Hoehn, Katharina ;
Mesen-Ramirez, Paolo ;
Bachmann, Anna ;
Lorenzen, Stephan ;
Roeder, Thomas ;
Metwally, Nahla Galal ;
Bruchhaus, Iris .
MICROORGANISMS, 2020, 8 (02)
[48]   Cytoadhesion of Plasmodium falciparum-infected erythrocytes and the infected placenta:: a two-way pathway [J].
Costa, F. T. M. ;
Avril, M. ;
Nogueira, P. A. ;
Gysin, J. .
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2006, 39 (12) :1525-1536
[49]   Micromolar Dihydroartemisinin Concentrations Elicit Lipoperoxidation in Plasmodium falciparum-Infected Erythrocytes [J].
Skorokhod, Oleksii ;
Valente, Elena ;
Mandili, Giorgia ;
Ulliers, Daniela ;
Schwarzer, Evelin .
ANTIOXIDANTS, 2023, 12 (07)
[50]   METABOLISM OF GLUTAMINE IN ERYTHROCYTES INFECTED WITH THE HUMAN MALARIA PARASITE - PLASMODIUM-FALCIPARUM [J].
VILMONT, M ;
AZOULAY, M ;
FRAPPIER, F .
ANNALES DE PARASITOLOGIE HUMAINE ET COMPAREE, 1990, 65 (04) :162-166