Plasmodium falciparum: a simplified technique for obtaining singly infected erythrocytes

被引:0
作者
Manoj K. Puthia
Kevin S. W. Tan
机构
[1] National University of Singapore,Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Faculty of Medicine
来源
Parasitology Research | 2005年 / 95卷
关键词
Malaria; Plasmodium Falciparum; Infected Erythrocyte; Thin Blood Smear; Rotation Culture;
D O I
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学科分类号
摘要
We report the development of a simple technique involving 15 ml polypropylene tubes and a rotatory incubator for obtaining erythrocytes singly infected with Plasmodium falciparum. This technique will be useful for cloning of the parasite. Our finding that P. falciparum merozoite invasion is inhibited during rotation suggests that this method may also be useful for the study of parasite-erythrocyte interactions under dynamic circulatory conditions.
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页码:176 / 178
页数:2
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