Comparison of three selectable marker genes for transformation of tall fescue (Festuca arundinacea Schreb.) plants by particle bombardment

被引：0
作者：
Danfeng Long
Xueli Wu
Zhimin Yang
Ingo Lenk
Klaus Kristian Nielsen
Caixia Gao
机构：
[1] Lanzhou University,School of Life Science
[2] Nanjing Agricultural University,College of Horticulture
[3] DLF-Trifolium Ltd.,Research Division
[4] Institute of Genetics and Developmental Biology,The State Laboratory of Plant Cell and Chromosome Engineering
[5] Chinese Academy of Sciences,undefined
来源：
In Vitro Cellular & Developmental Biology - Plant | 2011年 / 47卷
关键词：
Tall fescue (; Schreb.); Particle bombardment; Selectable markers; Herbicide; Antibiotic;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A variety of selection systems have been developed for transformation of forage crops. To compare the most frequently used systems, we tested three selectable marker genes for their selection efficiency under four selection procedures for the production of transgenic tall fescue. Embryogenic calluses initiated from mature embryos were bombarded with three constructs containing either the phosphinothricin acetyltransferase (bar) gene, the hygromycin phosphotransferase (hpt) gene or the neomycin phosphotransferase II (nptII) gene. Transformation efficiency was strongly influenced by the selectable marker gene, selection procedure and genotype. The highest transformation efficiency was observed using the bar gene in combination with bialaphos. Average transformation efficiencies with bialaphos, phosphinothricin (glufosinate), hygromycin and paromomycin selection across the two callus lines used in the experiments were 9.4%, 4.4%, 5.2% and 1.6%, respectively. Southern blot analysis revealed the independent nature of the tested transgenic plants and a complex transgene integration pattern with multiple insertions.
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页码：658 / 666
页数：8
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共 119 条
  • [1] Altpeter F.(2005)Particle bombardment and the genetic enhancement of crops: myths and realities Mol Breeding 15 305-327
  • [2] Baisakh N.(2000)Rapid production of transgenic turfgrass ( J Plant Physiol 157 441-448
  • [3] Beachy R.(2000) L.) plants Mol Breeding 6 519-528
  • [4] Altpeter F(2000)Generation of large numbers of independently transformed fertile perennial ryegrass ( Plant Cell Rep 19 1084-1089
  • [5] Xu J(1998) L.) plants of forage- and turf-type cultivars Plant Sci 138 229-244
  • [6] Altpeter F(1996)Production of transgenic tall fescue and red fescue plants by particle bombardment of mature seed-derived highly regenerative tissues Trans Res 5 213-218
  • [7] Xu J(1995)Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism Plant Sci 108 63-70
  • [8] Ahmed S(1994)Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledoneous plants Pant Cell, Tissue and Organ Culture 36 1-7
  • [9] Cho MJ(2005)The effect of selection pressure on transformation frequency and copy number in transgenic plants of tall fescue ( Plant Sci 168 1453-1458
  • [10] Ha CD(2006) Schreb.) Plant Cell Rep 25 19-25
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