Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19

被引：0

作者：

Fengjie Cui

Yin Li

Zhiqiang Liu

Hui Zhao

Lifeng Ping

Liying Ping

Yinan Yang

Yaping Xue

Lijiao Yan

机构：

[1] Jiangsu University,School of Food and Biotechnology

[2] North Dakota State University,Department of Plant Science

[3] Zhejiang University of Technology,Institute of Bioengineering

[4] Polytechnic Institute of New York University,Department of Chemical and Biological Science, NSF

[5] Six Metrotech Center,I/UCRC Center for Biocatalysis and Bioprocessing of Macromolecules

[6] North Dakota State University,Department of Statistics

[7] Zhejiang Academy of Agricultural Sciences,Institute of Quality and Standard for Agricultural Products

[8] Jilin Agricultural University,College of Horticulture

[9] Zhejiang University,College of Life Sciences

来源：

World Journal of Microbiology and Biotechnology | 2009年 / 25卷

关键词：

Identification; ZH-19; Response surface methodology; Optimization; Xylanase;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The objective of this study was to maximize production of xylanase by a newly isolated strain Penicillium thiersii ZH-19. Response surface methodology was employed to study the effects of significant factors such as pH, temperature, xylan concentration, and cultivation time, on the production of xylanase by Penicillium thiersii ZH-19. The optimal fermentation parameters for enhanced xylanase production were found to be pH 7.72, temperature 24.8°C, xylan 13.2 g l−1 and the fermentation time 125.8 h. The model predicted a xylanase activity of 75.24 U ml−1. Verification of the optimization showed that the maximum xylanase production reached 73.50 U mL−1 in the flask experiments and 80.23 U mL−1 in the scale of 15-L fermenter under the optimal condition.

引用

页码：721 / 725

页数：4

共 54 条

[1] Box GE(1960)Some new three level designs for the study of quantitative variable Technometrics 2 456-475
[2] Behnken DW(1997)Purification and characterization of a xylanase from Enzyme Microb Technol 21 91-96
[3] Chen C(2006) for xylooligosaccharide production Bioresour Technol 97 1209-1216
[4] Chen JL(1994)Optimization of the medium composition for production of mycelial biomass and exo-polymer by New Phytol 126 525-532
[5] Lin TY(1997) GF9801 using response surface methodology Lett Appl Microbiol 24 389-392
[6] Cui FJ(1993)Identification of the ectomycorrhizal basidiomycete tylospora-fibrillosa donk by RFLP analysis of the PCR-amplified ITS and IGS regions of ribosomal DNA Crit Rev Biotechnol 13 151-172
[7] Li Y(2005)Xylanolytic activity of commercial juice-processing enzyme preparations J Am Soc Brew Chem 63 171-176
[8] Xu ZH(2007)Lignocellulose biotechnology: current and future prospects J Food Sci 72 320-329
[9] Sun K(2007)Characterization of the enzymatic degradation of arabinoxylans in grist containing wheat malt using response surface methodology Biochem Eng J 34 82-86
[10] Tao WY(2007)Application of statistical experimental design to optimize culture requirements of a novel Process Biochem 42 889-894

← 1 2 3 4 5 6 →