Genome-wide survey and expression analysis of the stress-associated protein gene family in desert poplar, Populus euphratica

被引:0
作者
Huixia Jia
Jianbo Li
Jin Zhang
Yiqiu Ren
Jianjun Hu
Mengzhu Lu
机构
[1] Nanjing Forestry University,Co
[2] Chinese Academy of Forestry,Innovation Center of Sustainable Forestry in Southern China
来源
Tree Genetics & Genomes | 2016年 / 12卷
关键词
Stress-associated proteins; Gene family; Expression analysis; Gene structure; Phylogenetic analysis;
D O I
暂无
中图分类号
学科分类号
摘要
Stress-associated proteins (SAPs) are a novel class of zinc finger proteins that extensively participate in abiotic stress responses. To date, no overall analysis and expression profiling of SAP genes in woody plants have been reported. Populus euphratica is distributed in desert regions and is extraordinarily adaptable to abiotic stresses. Thus, it is regarded as a promising candidate for studying abiotic stress resistance mechanisms of woody plants. In this study, 18 non-redundant SAP genes were identified from the genome of P. euphratica using basic local alignment search tool algorithms and functional domain verification. Among these 18 PeuSAP genes, 15 were intronless. To investigate the evolutionary relationships of SAP genes in P. euphratica and other Salicaceae plants, phylogenetic analyses were performed. Subsequently, the expression profiles of the 18 PeuSAP genes were analyzed in different tissues and under various stresses (drought, salt, heat, cold, and abscisic acid (ABA) treatment) using quantitative real-time PCR. Tissue expression analysis indicated that PeuSAPs showed no tissue specificity. PeuSAPs were induced by multiple abiotic stresses, especially drought, salt, and heat stresses, perhaps because of abundant cis-acting heat shock elements and drought-inducible elements in the promoter regions of the PeuSAPs. Moreover, single nucleotide polymorphisms (SNPs) variant analysis revealed many synonymous and non-synonymous SNPs in PeuSAP genes, but the zinc finger structure was conserved during evolution. These results provide an overview of the SAP gene family in P. euphratica and a reference for further functional research on PeuSAP genes.
引用
收藏
相关论文
共 289 条
  • [1] Alfaro RI(2014)The role of forest genetic resources in responding to biotic and abiotic factors in the context of anthropogenic climate change For Ecol Manag 333 76-87
  • [2] Fady B(2008)Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes Afr J Biotechnol 7 2341-2352
  • [3] Vendramin GG(2012)Expression of the Plant Cell Environ 35 626-643
  • [4] Dawson IK(2000) AlSAP gene in rice confers broad tolerance to abiotic stresses through maintenance of photosynthesis Biochem Pharmacol 60 1143-1151
  • [5] Fleming RA(2007)A20 and A20-binding proteins as cellular inhibitors of nuclear factor-κB-dependent gene expression and apoptosis Diabetes 56 499-505
  • [6] Sáenz-Romero C(2011)Tag polymorphisms at the Int J Biochem Cell B 43 1612-1620
  • [7] Lindig-Cisneros RA(2012) ( Planta 236 567-577
  • [8] Murdock T(2014)) locus are associated with lower gene expression and increased risk of coronary artery disease in type 2 diabetes BMC Genomics 15 326-1440
  • [9] Vinceti B(2014)AWP1 binds to tumor necrosis factor receptor-associated factor 2 (TRAF2) and is involved in TRAF2-mediated nuclear factor-kappaB signaling Plant Cell Rep 33 1425-13
  • [10] Navarro CM(2011)Overexpression of a PLoS One 6 1-2978