Comparison of the Ligase Chain Reaction with Solid and Liquid Culture Media for Routine Detection of Mycobacterium tuberculosis in Nonrespiratory Specimens

被引：0

作者：

J. J. Palacios

J. Ferro

N. Ruiz Palma

S. G. Roces

H. Villar

J. Rodríguez

P. Prendes

机构：

[1] Servicio de Microbiología,

[2] Hospital San Agustín,undefined

[3] Camino de Heros 4,undefined

[4] E-33400 Avilés,undefined

[5] Asturias,undefined

[6] Spain,undefined

[7] Servicio de Medicina Interna,undefined

[8] Hospital Nuestra Señora de Los Reyes,undefined

[9] Valverde,undefined

[10] el Hierro,undefined

[11] Canarias,undefined

[12] Spain,undefined

[13] Servicio de Inmunología,undefined

[14] Hospital Central de Asturias,undefined

[15] Oviedo,undefined

[16] Asturias,undefined

[17] Spain,undefined

来源：

European Journal of Clinical Microbiology and Infectious Diseases | 1998年 / 17卷

关键词：

Tuberculosis; Liquid Medium; Liquid Culture; Mycobacterium Tuberculosis; Solid Medium;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The aim of this study was to compare the results of a commercial assay based on the ligase chain reaction [(LCR) LCx Probe System MTB; Abbott, USA] with those of culture in liquid medium (Septi-Chek AFB; Becton-Dickinson, USA) and culture on the egg-based Löwenstein-Jensen solid medium for the direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens. The results were analyzed according to the standard definition of a true-positive result. Two hundred thirty-five nonrespiratory samples routinely submitted to rule out tuberculosis were analyzed. All samples were smear-negative. Mycobacterial growth in either culture medium was detected in 18 (7.6%) specimens: Mycobacterium tuberculosis was recovered from seven (38.9%) specimens cultured on Löwenstein-Jensen medium and from 18 (100%) specimens cultured in Septi-Chek AFB. The LCR protocol was positive in 22 specimens. None of the LCR-negative controls showed positive results. All samples positive by culture on Löwenstein-Jensen medium were positive by culture in liquid medium and by the LCR assay. However, Mycobacterium tuberculosis was detected by culture in liquid medium in two specimens that were negative by the LCR assay, whereas six specimens negative by culture in liquid medium were positive by the LCR protocol; three of these were identified as true-positive results of the LCR assay. The sensitivity, specificity, and positive and negative predictive values were 33.3%, 100%, 100%, and 93.8%, respectively, for Löwenstein-Jensen medium; 85.7%, 100%, 100%, and 98.6% for the liquid medium; and 90.4%, 98.5%, 86.3%, and 99% for the LCR assay. These findings indicate that the LCR assay may be a valid method of high diagnostic yield for direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens.

引用

页码：767 / 772

页数：5