Sunflower Nitrate Reductase: Purification and Characterization

被引：0
作者：
Debasis Pattanayak
Sukumar R. Chatterjee
机构：
[1] Indian Agricultural Research Institute,Division of Biochemistry
[2] Indian Agricultural Research Institute,Nuclear Research Laboratory
[3] Central Potato Research Institute,Division of Crop Physiology & Biochemistry
来源：
Journal of Plant Biochemistry and Biotechnology | 1999年 / 8卷
关键词：
nitrate reductase; sunflower; blue Sepharose CL-6B; molecular weight; sub-unit; pH optima; ferric citrate reductase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A single isoform, NADH: nitrate reductase (NR), was purified 500 folds from sunflower leaves by affinity chromatography on Blue Sepharose CL-6B. Purified NR had a pH optima of 7.25 and a molecular weight of 210 kD. In SDS-PAGE, two bands of 47 and 56 kD were obtained. NADH: ferric citrate reductase activity was copurified with NR with a specific activity of 2. The Vmax of NADH: ferric citrate reductase was 8.69 units mg-1 protein and the apparent Km for ferric citrate was 0.435 mM.
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页码：41 / 45
页数：4
相关论文
共 48 条
[1]  
Hageman R.H.(1980)undefined Methods Enzymol 59 270-undefined
[2]  
Reed A.J.(1992)undefined Physiol Plant 84 616-undefined
[3]  
Hoff T.(1983)undefined Biochem Biophys Res Commun 114 1182-undefined
[4]  
Stummann B.M.(1978)undefined Plant Physiol 61 611-undefined
[5]  
Henningsen K.W.(1985)undefined J Biol Chem 260 3380-undefined
[6]  
Redinbaugh M.G.(1987)undefined Plant Physiol 84 654-undefined
[7]  
Campbell W.H.(1998)undefined Indian J Exp Biol 36 644-undefined
[8]  
Campbell W.H.(1988)undefined Can J Bot 66 1639-undefined
[9]  
Smarrelli J.(1970)undefined Nature 227 680-undefined
[10]  
Redinbaugh M.G.(1990)undefined Methods Enzymol 182 425-undefined
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