Functional Identification of H+-ATPase and Na+/H+ Antiporter in the Plasma Membrane Isolated from the Root Cells of Salt-Accumulating Halophyte Suaeda altissima

被引:0
作者
R. V. Lun'kov
I. M. Andreev
N. A. Myasoedov
G. F. Khailova
E. B. Kurkova
Yu. V. Balnokin
机构
[1] Russian Academy of Sciences,Timiryazev Institute of Plant Physiology
来源
Russian Journal of Plant Physiology | 2005年 / 52卷
关键词
Suaeda altissima; salt-accumulating halophyte; root; plasma membrane vesicles; H; -ATPase; Na; /H; antiporter;
D O I
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中图分类号
学科分类号
摘要
A membrane fraction enriched in plasma membrane (PM) vesicles was isolated from the root cells of a salt-accumulating halophyte Suaeda altissima (L.) Pall. by means of centrifugation in discontinuous sucrose density gradient. The PM vesicles were capable of generating ΔpH at their membrane and the transmembrane electric potential difference (Δψ). These quantities were measured with optical probes, acridine orange and oxonol VI, sensitive to ΔpH and Δψ, respectively. The ATP-dependent generation of ΔpH was sensitive to vanadate, an inhibitor of P-type ATPases. The results contain evidence for the functioning of H+-ATPase in the PM of the root cells of S. altissima. The addition of Na+ and Li+ ions to the outer medium resulted in dissipation of ΔpH preformed by the H+-ATPase, which indicates the presence in PM of the functionally active Na+/H+ antiporter. The results are discussed with regard to involvement of the Na+/H+ antiporter and the PM H+-ATPase in loading Na+ ions into the xylem of S. altissima roots.
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页码:635 / 644
页数:9
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