Resistance to different generations of quinolones in Streptococcus pneumoniae strains isolated from hospitals in Shiraz

被引：2
作者：
Kargar M. [1 ]
Jahromi F.M. [1 ]
Doosti A. [2 ]
Mohammadalipour Z. [1 ]
Lorzadeh S. [2 ]
机构：
[1] Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom
[2] Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord
来源：
Comparative Clinical Pathology | 2015年 / 24卷 / 3期
关键词：
Antibiotic resistance; lytA; Quinolones; Streptococcus pneumoniae;
D O I：
10.1007/s00580-014-1937-3
中图分类号：
学科分类号：
摘要：
Streptococcus pneumoniae is a leading cause of respiratory tract infections, including pneumonia. In recent years, due to the incidence of resistance to commonly used antibiotics, quinolones have been taken into consideration in treatment of S. pneumoniae. The present study aimed to investigate the quinolone resistance rate among pneumococcus isolates from Shiraz. This cross-sectional-descriptive study was performed on 45 strains of S. pneumoniae isolated from clinical samples. Initially, using biochemical tests and PCR technique with specific primers of lytA gene, S. pneumoniae strains were identified. Subsequently, antibiotic resistance to quinolones was evaluated by CLSI criteria. Among 45 strains isolated from clinical specimen, resistance to nalidixic acid, ciprofloxacin, ofloxacin, norfloxacin and levofloxacin were estimated 88.22, 73.43, 53.33, 48.88 and 42.22 %, respectively. The highest frequency of resistance corresponded to the people with pneumonia. Besides, the greatest resistance to all antibiotics related to the age group of 31–40. Statistical analysis indicated no significant association between antibiotic resistance and disease type or age groups. In concurrence with other investigations, our results showed that the level of resistance to quinolones is increasing in Shiraz. Hence, the quinolone resistance in this region is a serious issue; therefore, the regional pattern of antimicrobial resistance on therapeutic regimes must be taken into consideration. © 2014, Springer-Verlag London.
引用
收藏
页码：533 / 536
页数：3
相关论文
共 19 条
  • [1] Adam H.J., Schurek K.N., Nichol K.A., Hoban C.J., Baudry T.J., Laing N.M., Molecular characterization of increasing fluoroquinolone resistance in Streptococcus pneumoniae isolates in in Canada, 1997 to 2005, Antimicrob Agents Chemother, 51, 1, pp. 198-207, (2007)
  • [2] Balsalobre L., Ferrandiz M.J., Alba G., De la Campa A.G., Nonoptimal DNA topoisomerases allow maintence of supercoiling level and improve fitness of Streptococcus pneumoniae, Antimicrob Agents Chemother, 55, 3, pp. 1079-1105, (2011)
  • [3] El-Mahmood A., Isa H., Mohammed, Tirmidhi A.B., Antimicrobial susceptibility of some respiratory tract pathogens to commonly used antibiotics at the Specialist Hospital, Yola, Adamawa State, Nigeria, J Clin Med Res, 2, 8, pp. 135-142, (2010)
  • [4] Foroumadi A.R., Haghighat P., Emami S., Moshafi M.H., Synthesis of new piperazinyl quinolones and investigation of their in vitro antibacterial activities, J Kerman Univ Med Sci, 5, 3, pp. 103-109, (1998)
  • [5] Fukushima K.Y., Yanagihara K., Hirakata Y., Sugahara K., Morinaga Y., Kohno S., Rapid identification of penicillin resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by us of a novel real-time multiplex PCR assay, J Clin Microbiol, 46, 7, pp. 2384-2388, (2008)
  • [6] Fuller J.D., Low D.E., A review of Streptococcus pneumoniae infection treatment failures associated with fluoroquinolone resistance, Clin Infect Dis, 41, 1, pp. 118-121, (2005)
  • [7] Kargar M., Baghernejad M., Ghorbani-daline S., Hashemizade Z., Evaluation of molecular mechanisms resistance to macrolides by Streptococcus pneumoniae strains isolated from Nemazee and Shahid Faghihi Hospitals in Shiraz, Sci J Kurdistan Univ Med Sci, 16, 4, pp. 83-91, (2012)
  • [8] Lesher G.Y., Fredrich E.J., Greott M.D., Bailey J.H., Brundach P.R., 1,8-Naphthyridine derivatives, a new class of chemotherapeutic agents, J Med Chem, 91, pp. 1063-1068, (1962)
  • [9] Linares J., Ardauny C., Pallares R., Fenoll A., Changes in antimicrobial resistance serotypes and genotypes in Streptococcus pneumoniae over a 30-year period, Clin Microbiol Infect, 16, 5, pp. 402-410, (2010)
  • [10] Mandell G.L., Goodman and Gilmans the pharmacological basis of therapeutics, (1990)
12